Detection of a human CFC with a high proliferative potential
IK McNiece, FM Stewart, DM Deacon, DS Temeles, KM Zsebo, SC Clark and PJ Quesenberry
School of Medicine, University of Virginia, Charlottesville.
Colony forming cells (CFC) with high proliferative potential have been
detected in nutrient agar cultures of human bone marrow cells containing
recombinant human interleukin-3 (IL-3) and granulocyte macrophage colony
stimulating factor (GM-CSF). These CFC were detected by the formation of
large colonies with diameters greater than 0.5 mm and containing
approximately 50,000 cells after 28 days incubation. The incidence of these
CFC was only two in 100,000 normal bone marrow cells; however, bone marrow
from patients treated with 5-fluorouracil contained up to sevenfold higher
numbers of these CFC. The characteristics of these CFC,
multifactor-responsive progenitors with high proliferative potential,
requiring a prolonged growth period in culture and showing a relative
preservation in marrow from individuals pretreated with 5-fluorouracil, are
consistent with a human cell type equivalent to the primitive murine
progenitor termed HPP-CFC.
Volume 74,
Issue 2,
pp. 609-612,
08/01/1989
Copyright © 1989 by The American Society of Hematology
