An antifibrin monoclonal antibody useful in immunoscintigraphic detection
of thrombi
MN Wasser, PW Koppert, JW Arndt, JJ Emeis, RI Feitsma, EK Pauwels and W Nieuwenhuizen
Gaubius Institute TNO, Leiden, The Netherlands.
Balb/c mice were immunized with human plasmin-generated fibrinogen
degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma
cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs)
with a strong reactivity with human fibrin and only a weak reactivity with
fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of
rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 kappa-type
and appear to be directed against a conformation-dependent epitope in the
D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that
Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to preformed
plasma clots in a plasma milieu, even in the presence of high
concentrations of heparin. Clot localization experiments in rabbits and
rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for
thrombus imaging in vivo.
Volume 74,
Issue 2,
pp. 708-714,
08/01/1989
Copyright © 1989 by The American Society of Hematology
