SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ehrlich, G. D. Articles by Papsidero, L. Search for Related Content PubMed PubMed Citation Articles by Ehrlich, G. D. Articles by Papsidero, L. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Detection of anti-HTLV-I Tax antibodies in HTLV-I enzyme-linked immunosorbent assay-negative individuals GD Ehrlich, JB Glaser, MA Abbott, DJ Slamon, D Keith, M Sliwkowski, J Brandis, E Keitelman, Y Teramoto and L Papsidero Department of Medicine, SUNY HSC Syracuse, 13210. The HTLV-I tax gene protein (Tax) is not packaged within the mature viral particle from which the proteins for the commercially available enzyme-linked immunosorbent assay (ELISA) are derived. Screening of 162 individuals within a cohort of white intravenous (IV) drug abusers, previously identified as having an increased incidence of HTLV-I infection, demonstrated that seven of them had antibodies to the HTLV-I Tax protein but tested negative in HTLV-I ELISAs and Western blots prepared from purified virion proteins. Three out of 35 individuals in other behaviorally defined high-risk groups also displayed this limited pattern of reactivity to HTLV-I proteins. The presence of the anti-HTLV- I p40/Tax antibodies was determined by radioimmunoprecipitation assay (RIPA), which also revealed low levels of anti-env reactivity. The specificity of the anti-p40 reactivity was confirmed on specific Tax ELISAs and Western blots prepared from recombinantly produced Tax. In vitro gene amplification by the polymerase chain reaction (PCR) was used to establish the presence of sequences homologous to HTLV-I proviral DNA in four/four of these HTLV-I ELISA negative, Tax ELISA/Tax western blot/RIPA positive individuals. These data suggest that the true incidence of HTLV-I infection within high-risk cohorts is greater than previously reported. Volume 74, Issue 3, pp. 1066-1072, 08/15/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. A. Youngberg, A. Karnad, M. P. Busch, E. Murphy, G. Nemo, D. Zucker-Franklin, and B. A. Pancake More on HTLV tax and Mycosis Fungoides N. Engl. J. Med., December 30, 1993; 329(27): 2035 - 2036. [Full Text]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020