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Proliferative potential of degranulated murine peritoneal mast cells
A Kuriu, S Sonoda, Y Kanakura, K Jozaki, A Yamatodani and Y Kitamura
Department of Pathology, Osaka University Medical School, Japan.
The fate of mast cells after degranulation was investigated. Purified
peritoneal mast cells of WBB6F1-+/+ mice were sensitized with monoclonal
anti-dinitrophenol (DNP) IgE antibodies and stimulated with DNP conjugated
with human serum albumin. Mast cells were vitally stained with neutral red,
and highly degranulated mast cells were identified under a phase-contrast
microscope and individually picked up with the micromanipulator. When these
highly degranulated mast cells were individually plated in methylcellulose,
their potential to produce a cluster or a colony was comparable to that of
morphologically intact mast cells. Moreover, when highly degranulated mast
cells were injected into the skin of genetically mast cell-deficient
WBB6F1-W/Wv mice, the proportion of injection sites at which mast cell
clusters appeared was comparable to the value observed when morphologically
intact mast cells were injected. The present result indicates that
proliferative potential of mast cells is not reduced by their
degranulation.
Volume 74,
Issue 3,
pp. 925-929,
08/15/1989
Copyright © 1989 by The American Society of Hematology
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