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Macrophage colony-stimulating factor (CSF-1) is expressed by spontaneously
outgrown EBV-B cell lines and activated normal B lymphocytes
G Reisbach, J Sindermann, JP Kremer, L Hultner, H Wolf and P Dormer
Gesellschaft fur Strahlen- und Umweltforschung, Institut fur Experimentelle
Hamatologie, Universitat Munchen, FRG.
Human B lymphocytes activated by mitogens or infected by Epstein Barr virus
(EBV) have previously been shown to release colony-stimulating activity
(CSA) supporting the growth of normal human bone marrow progenitors. We
established five different human EBV-B cell lines spontaneously outgrown
from nonmalignant peripheral blood cells and long-term bone marrow
cultures. CSA derived from all of these lines induces the growth of murine
macrophage colonies, whereas virtually no human bone marrow cell
progenitors were stimulated. As observed in the tumor cell line MIA PaCa-2,
a 4.3-kilobase (kb) transcript was detected in all cases using a human
colony-stimulating factor (CSF)-1 probe. Expression of this transcript can
be further stimulated within three hours upon addition of phorbol myristate
acetate (PMA). The highly purified native protein exerting macrophage
colony-stimulating activity (M-CSA) exhibits a molecular size of
approximately 75 to 97 Kd in sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SDS-PAGE). The identity of EBV-B cell derived M-CSA with
human urinary CSF-1 was confirmed by a complete neutralization of
macrophage CSA by an antihuman urinary CSF-1 antiserum. Normal human B
lymphocytes purified from tonsils or from mononuclear blood cells also
express CSF-1 upon stimulation with Staphylococcus aureus Cowan I. No CSF-1
expression, however, could be detected in normal resting B lymphocytes or
in the Burkitt lymphoma cell line RAJI.
Volume 74,
Issue 3,
pp. 959-964,
08/15/1989
Copyright © 1989 by The American Society of Hematology
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