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Sequential loss of CD34 and class II MHC antigens on purified cord blood
hematopoietic progenitors cultured with IL-3: characterization of CD34-,
HLA-DR+ cells
C Caux, C Favre, S Saeland, V Duvert, P Mannoni, I Durand, JP Aubry and JE de Vries
UNICET, Laboratory for Immunological Research, Dardilly, France.
The expression of class II MHC and CD34 antigens on human cord blood
hematopoietic progenitor cells (HPC) was investigated upon culturing in the
presence of interleukin-3 (IL-3). HPC isolated by "panning" according to
their expression of CD34 coexpressed HLA-DR and HLA-DP, and the majority of
the CD34+ HPC also expressed HLA-DQ. In the presence of IL-3, the
expression of CD34 and class II MHC antigens was found to be gradually lost
in culture. Loss of CD34 expression preceded loss of HLA-DR expression.
After eight days of culture, CD34-, HLA-DR+ blast cells were obtained that
strongly proliferated in response to IL- 3, GM-CSF, G-CSF, and M-CSF, and
that had the capacity to generate macrophage and granulocyte colonies.
After ten days of culture in IL-3, a population of CD34- cells that
expressed low levels of HLA-DR (HLA- DRlo) was obtained by FACS-sorting.
These CD34-, HLA-DRlo cells lacked colony-forming activity while the
population expressing high levels of HLA-DR (HLA-DRhi) contained great
numbers of colony-forming cells, and proliferated stronger in response to
CSFs than the HLA-DRlo fraction. Finally CD34-, HLA-DR- cells that appeared
later in the cultures (14 to 16 days) represented more differentiated cells
with only marginal proliferative and no clonogenic capacity. These data
indicate that whereas CD34 expression is associated with the multilineage
potential of the HPC, HLA-DR expression correlates with overall
proliferative capacity of hematopoietic cells during culture in IL-3.
Volume 74,
Issue 4,
pp. 1287-1294,
09/01/1989
Copyright © 1989 by The American Society of Hematology
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