CML-T1: a cell line derived from T-lymphocyte acute phase of chronic
myelogenous leukemia
K Kuriyama, RP Gale, M Tomonaga, S Ikeda, E Yao, I Klisak, K Whelan, H Yakir, M Ichimaru and RS Sparkes
Department of Hematology, Nagasaki University School of Medicine, Japan.
Most data suggest that malignant transformation in chronic myelogenous
leukemia (CML) occurs in hematopoietic stem cell that is the progenitor of
myelopoiesis and of B but not T lymphopoiesis. We established a T- lymphoid
cell line (CML-T1) from a person with Ph-chromosome-negative CML in acute
phase. Evidence of its T-lymphocyte origin includes the pattern
cytochemical reactivity, reactivity with anti-T-cell monoclonal antibodies
(MoAbs), and rearrangement of the beta-T-cell receptor (TCRB) gene. CML-T1
cells have features of type IV thymocytes. Cytogenetic analyses indicate a
47,XX, del(11), t(6;7)(q23;q24), +mar karyotype. CML-T1 cells exhibit
molecular changes typical of CML, including translocation of the ABL
protooncogene from chromosome 9 to 22, rearrangement of the BCR gene, and
transcription of a chimeric BCR- ABL messenger RNA (mRNA). The ABL
insertion on chromosome 22 appears interstitial, similar to other cases of
Ph-chromosome-negative CML. These data clearly indicate that T cells can be
involved in acute-phase CML. CML-T1 should be useful in studying this
process as well as that underlying Ph-chromosome-negative CML.
Volume 74,
Issue 4,
pp. 1381-1387,
09/01/1989
Copyright © 1989 by The American Society of Hematology
