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Nuclear oncoprotein expression as a function of lineage, differentiation
stage, and proliferative status of normal human hematopoietic cells
MB Kastan, KD Stone and CI Civin
Johns Hopkins Oncology Center, Baltimore, MD.
Relative levels of the nuclear oncoproteins c-myb, c-myc, and c-fos were
determined in selected subpopulations of normal human bone marrow (BM)
cells using a flow cytometric assay which simultaneously detects a
cell-surface antigen (as a marker of lineage and stage of maturation) and
levels of an intracellular protein. At least two monoclonal antibodies
directed against each oncoprotein and specific peptide inhibition controls
were used for these determinations. Hematopoietic progenitor cells (CD34+)
express the highest levels of c-myb and c-myc, whereas c-fos levels in
CD34+ progenitor cells are similar to c-fos levels in mature monocytes and
granulocytes. Granulocytes are the only hematopoietic cells examined which
do not express detectable levels of c-myb and c-myc. The levels of these
oncoproteins in these normal, unstimulated BM cell populations were more
closely linked to lineage and maturation stage than to the proliferative
status of the given population, as determined by either DNA staining or
expression of the cell-cycle specific nuclear protein, Ki67. This flow
cytometric assay helps in interpreting the significance of oncoprotein
levels in leukemia cells by allowing direct comparisons of a leukemia with
the phenotypically similar "normal counterpart control" cell population in
normal BM.
Volume 74,
Issue 5,
pp. 1517-1524,
10/01/1989
Copyright © 1989 by The American Society of Hematology
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