Different repopulation profile between erythroid and nonerythroid
progenitor cells in genetically anemic W/Wv mice after bone marrow
transplantation
T Nakano, N Waki, H Asai and Y Kitamura
Division of Cancer Pathology, Osaka University Medical School, Japan.
Repopulation kinetics of erythrocytes and neutrophils and replacement of
hematopoietic progenitors were studied in genetically anemic (WB x
C57BL/6)F1-W/Wv (WBB6F1-W/Wv) hosts after bone marrow transplantation from
C57BL/6-bgJ/bgJ or C57BL/6-bgJ/bgJ;Pgk-1a/Y mice. Electrophoretic pattern
of hemoglobin was used as a marker of donor-type erythrocytes, giant
granules of bgJ/bgJ mice as a marker of donor-type neutrophils, and A-type
phosphoglycerate kinase-1 (PGK-1) as a marker of hematopoietic colonies
produced by donor-derived progenitor cells. Repopulation of donor-type
erythrocytes was significantly faster than that of donor-type neutrophils.
Moreover, the extent of replacement was greater for erythroid progenitor
cells than for nonerythroid progenitor cells. When nonirradiated
WBB6F1-W/Wv mice with B-type PGK-1 received 10(5) bone marrow cells from
C57BL/6-bgJ/bgJ;Pgk-1a donors, only approximately 20% replacement of
erythroid progenitor cells gave rise to total reconstitution of
erythrocytes. The present result suggests that normal multipotential stem
cells may preferentially differentiate into erythroid lineage cells in
anemic WBB6F1-W/Wv hosts and that normal erythroid progenitor cells may
suppress the differentiation of erythroid progenitors of WBB6F1-W/Wv hosts.
Volume 74,
Issue 5,
pp. 1552-1556,
10/01/1989
Copyright © 1989 by The American Society of Hematology
