Suppressive effect of Sl/Sld mouse embryo-derived fibroblast cell lines on
diffusible factor-dependent proliferation of mast cells
H Onoue, Y Ebi, H Nakayama, XM Ru, Y Kitamura and J Fujita
Department of Pathology, Osaka University Medical School, Japan.
Two modes of mast cell growth are present, one dependent on diffusible
growth factors (interleukins [IL] 3 and 4) and another dependent on contact
with fibroblasts. The 3T3 fibroblast cell lines derived from WCB6F1-+/+
mouse embryos supported the proliferation of cultured mast cells (CMC),
whereas the 3T3 fibroblast cell lines from WCB6F1-Sl/Sld mouse embryos did
not. To investigate the relationship between growth factor-dependent and
fibroblast-dependent growths of mast cells, we cocultured CMC and 3T3
fibroblasts in the presence of diffusible growth factors. WCB6F1-+/+ mouse
embryo-derived 3T3 cells did not affect the growth factor-dependent
proliferation of CMC, but WCB6F1-Sl/Sld mouse embryo-derived 3T3 cells
significantly suppressed the proliferation. Close cell-to-cell contact was
necessary for the suppression. The NWS1 fibroblast cell line was
established from the spleen cells of an adult WBB6F1-+/+ mouse. Although
the NWS1 cell line had no supporting effect on the proliferation of CMC in
the absence of diffusible growth factors, it did not suppress the
proliferation of CMC induced by the growth factors. The present result
suggests that a product of mutant Sl genes may be involved in the
suppressive activity of WCB6F1-Sl/Sld mouse embryo-derived 3T3 cells.
Volume 74,
Issue 5,
pp. 1557-1562,
10/01/1989
Copyright © 1989 by The American Society of Hematology
