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Loss of copper-zinc superoxide dismutase gene expression in differentiated
cells of myelo-monocytic origin
JH Auwerx, A Chait, G Wolfbauer and SS Deeb
Division of Metabolism, Endocrinology, and Nutrition, University of
Washington, Seattle.
Changes in the production of reactive oxygen species and total superoxide
dismutase activity have been observed during differentiation of some
hematopoietic cells. We therefore investigated whether the steady-state
level and rate of transcription of superoxide dismutase-1 (SOD-1) mRNA
change during terminal differentiation of the human leukemia cell lines
THP-1, HEL, and HL-60 into macrophages and/or granulocytes, respectively.
Macrophage differentiation is accompanied by a gradual decrease in both the
transcription rate (10x) and the steady-state level (6x) of SOD-1 mRNA. No
decrease was observed after treatment with the diacylglycerol analog 1,2
dioctanol-rac-glycerol (di- C8), which like phorbol 12-myristate 13-acetate
also activates protein kinase C but does not induce differentiation at the
concentration used. The same decrease in SOD-1 mRNA level was observed when
HL-60 cells were induced to differentiate into granulocytes by treatment
with dimethylsulfoxide. These data suggest that a decrease in SOD-1 mRNA to
almost undetectable levels accompanies differentiation of macrophages and
granulocytes.
Volume 74,
Issue 5,
pp. 1807-1810,
10/01/1989
Copyright © 1989 by The American Society of Hematology
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