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The fate of the open canalicular system in surface and suspension-
activated platelets
G Escolar, E Leistikow and JG White
Hospital Clinico y Provincial, Barcelona, Spain.
We have examined the movement of fibrinogen-gold (fgn-Au) complexes in
platelets activated in suspension and by surface contact. Fgn-Au probes did
not react with resting cells but were bound to the external membrane of
platelets in suspension 5 seconds after addition of 1 U/mL of thrombin. At
intervals over a period of 5 to 20 minutes, fgn-Au probes moved from the
cell surface to peripheral and then deep channels of the open canalicular
system (OCS). When platelets were surface activated by exposure to
carbon-stabilized, formvar-coated grids for 5 to 20 minutes and then
exposed to fgn-Au complexes for 5 minutes, probes were also observed in the
OCS. At 5 minutes, over 40% of the platelets had concentrated fgn-Au in
their OCS. Results after 10 minutes revealed 25% with gold-filled channels,
16% after 15 minutes, and 5% after 20 minutes. The decrease in frequency of
OCS staining correlated with the increasing frequency of spread platelets,
suggesting that tension produced by spreading may cause collapse of the OCS
or that the OCS may evaginate onto the platelet during spreading. To
evaluate the latter hypothesis, platelets were initially exposed to grids
for 5 minutes and then incubated with fgn-Au for intervals of 5 to 20
minutes. The frequency of platelets with fgn-Au concentrated in the OCS was
greatest at 5 minutes (44%) and decreased at the same rate as the frequency
of spread platelets increased. Only 14.7% of the cells contained fgn-Au in
the OCS after 20 minutes. These were primarily dendritic in form, while
fully spread platelets rarely contained an OCS filled with the probe. The
study indicates that fgn-Au particles are cleared to channels of the OCS
independent of the mechanism of platelet activation. Fgn-Au that has been
concentrated in the OCS at early stages of surface activation can be
externalized during platelet spreading but remain internalized in
suspension-activated cells. The OCS represents a membrane reservoir that
can be evaginated onto the platelet surface during interaction with
surfaces.
Volume 74,
Issue 6,
pp. 1983-1988,
11/01/1989
Copyright © 1989 by The American Society of Hematology
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