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An integrin receptor on normal and thrombasthenic platelets that binds
thrombospondin [see comments]
J Lawler and RO Hynes
Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115.
The members of the integrin family of membrane glycoprotein heterodimer
complexes function as cell surface receptors for adhesive proteins. We
report here on the identification of two integrins on the surface of human
platelets that bind to thrombospondin. When platelet membrane proteins are
radiolabeled with 125I-lactoperoxidase, solubilized in n- octylglucoside,
(Boehringer Mannheim Biochemicals, Indianapolis, IN), and applied to a
column of thrombospondin-Sepharose, both complexes are bound to the column
and specifically eluted with the peptide GRGDSP. One of these integrins,
glycoprotein (GP) IIb-IIIa, appears to bind relatively weakly. The second
integrin shares the same beta subunit (beta 3 or GPIIIa), but has a
distinct alpha subunit that comigrates with the alpha subunit (alpha v) of
the vitronectin receptor (VnR) on endothelial cells and reacts with a
monoclonal antibody, LM142, which was raised against an integrin from M21
melanoma cells. The alpha v beta 3 integrin is present on a variety of cell
types and appears to act as a receptor for thrombospondin on endothelial
and smooth muscle cells. On endothelial and M21 melanoma cells this
receptor is also involved in adhesion to fibrinogen, vitronectin, and von
Willebrand factor (vWF). The alpha v beta 3 integrin is present at
approximately equal levels on normal and thrombasthenic platelets, whereas
levels of GPIIb-IIIa are greatly reduced on thrombasthenic platelets. The
alpha v beta 3 integrin on thrombasthenic platelets also binds to
thrombospondin-Sepharose and can be eluted with the peptide GRGDSP. These
data indicate that the alpha v beta 3 integrin on platelets, endothelial
cells, and smooth muscle cells functions as an Arg-Gly-Asp (RGD)-dependent
receptor for thrombospondin.
Volume 74,
Issue 6,
pp. 2022-2027,
11/01/1989
Copyright © 1989 by The American Society of Hematology
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