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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Niemeyer, C. M. Articles by Nathan, D. G. Search for Related Content PubMed PubMed Citation Articles by Niemeyer, C. M. Articles by Nathan, D. G. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Hematopoiesis in vitro coexists with natural killer lymphocytes CM Niemeyer, CA Sieff, BR Smith, KA Ault and DG Nathan Division of Hematology and Pediatric Oncology, Children's Hospital, Boston, MA. The role of natural killer (NK) lymphocytes in the regulation of human hematopoiesis is controversial. NK-mediated inhibition of colony formation of hematopoietic progenitor cells has been irregularly reported for various cell lineages. In an effort to clarify such disparate findings, we studied the interaction of clearly defined NK and partially purified progenitor cell populations. Cell sorter purified CD16 positive blood NK cells and enriched autologous marrow progenitors were co-incubated at various lymphocyte to marrow cell ratios and then cultured in methylcellulose. There was no inhibition of myeloid, erythroid, or mixed colony formation. Similarly, activation of CD16 positive lymphocytes by interleukin-2 (IL-2) before co-incubation and co-culture did not result in inhibition of colony formation. Furthermore, in a newly designed assay system, we demonstrated that NK cells, which did not modulate colony-formation, remained capable of recognizing and killing rare K562 target cells seeded within the marrow cell population. Our results indicate that unstimulated and IL-2 activated isolated blood NK cells coexist with functioning autologous marrow progenitors in vitro. Volume 74, Issue 7, pp. 2376-2382, 11/15/1989 Copyright © 1989 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Binder, J. Fehr, H. Hengartner, and R. M. Zinkernagel Virus-induced Transient Bone Marrow Aplasia: Major Role of Interferon-{alpha}/{beta} during Acute Infection with the Noncytopathic Lymphocytic Choriomeningitis Virus J. Exp. Med., February 3, 1997; 185(3): 517 - 530. [Abstract] [Full Text] [PDF]

	Copyright © 1989 by American Society of Hematology         Online ISSN: 1528-0020