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Production of anti-B monoclonal antibodies (DBB.42, DBA.44, DNA.7, and
DND.53) reactive on paraffin-embedded tissues with a new B-lymphoma cell
line grafted into athymic nude mice
T al Saati, S Caspar, P Brousset, S Chittal, P Caveriviere, H Hounieu, N Dastugue, JB Idoipe, J Icart and C Mazerolles
Laboratoire d'Anatomie Pathologique CHU Purpan, Toulouse, France.
A new B-lymphoma cell line (DEAU-cell line) was established from a diffuse
large-cell lymphoma (centroblastic type) and was successfully grafted in
athymic nude mice. Monoclonal antibodies (MoAbs) were generated using
splenocytes of DEAU-tumor bearing mice. Before the fusion experiments,
cellular immunity of the mice bearing growing DEAU tumors was restored by
injection of spleen cells from conventional Balb/C mice. Spleen cells from
conventional Balb/C mice immunized with DEAU-cell line were also used for
the generation of MoAbs. Four MoAbs (DBB.42 and DBA.44 from normal Balb/C
mice, and DNA.7 and DND.53 from athymic nude mice) were investigated
because they identified B-cell- associated antigens not destroyed by
fixatives. DBB.42 recognized a pan- B cell-associated antigen (molecular
weight (mol wt) = 45 Kd). DBA.44 detected a B-cell antigen (mol wt not
determined) expressed on a subpopulation of B lymphocytes in the mantle
zone of lymphoid follicles. DNA.7 also defined a B-cell antigen (43 Kd)
mainly expressed on germinal center cells. Similarly, DND.53 recognized a
B-cell antigen (two bands of mol wt 20 Kd and 35 Kd, respectively) mainly
expressed on germinal center cells and mantle zone lymphocytes and
interdigitating reticulum cells in the paracortical area. Major differences
were found in the reactivities of these MoAbs on malignant lymphomas.
DBB.42 was positive with almost all B-cell lymphomas and some T-cell
lymphomas. Within the group of low-grade B-cell lymphomas, DBA.44 reacted
principally with hairy-cell leukemia. DNA.7 reacted mainly with high- grade
B-cell lymphomas with a weak positivity in low-grade B-cell lymphomas.
DND.53 reacted with all but one B-cell lymphoma, cells of histiocytosis X,
and Reed-Sternberg cells. These findings indicate that new MoAbs can be
generated by using spleen cells from athymic mice bearing human tumors as
well as by new lymphoid cell lines. The MoAbs so generated, as in the
present study, are deemed potentially useful for the recognition of B-cell
lymphomas in routine diagnostic histopathology. In addition, DND.53 could
be of value for the diagnosis of histiocytosis X and the detection of
Reed-Sternberg cells in Hodgkin's disease.
Volume 74,
Issue 7,
pp. 2476-2485,
11/15/1989
Copyright © 1989 by The American Society of Hematology
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