Interleukin-6 is not involved in the interleukin-1-induced production of
colony-stimulating factors by human bone marrow stromal cells and
fibroblasts
MR Schaafsma, WE Fibbe, J Van Damme, N Duinkerken, P Ralph, K Kaushansky, BW Altrock, R Willemze and JH Falkenburg
Department of Hematology, University Medical Center, Leiden, The
Netherlands.
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a role in
regulation of hematopoiesis. Because IL-6 is coinduced with colony-
stimulating factors (CSFs) by various cell types in response to stimulation
with IL-1, we investigated whether IL-6 is involved in the IL-1-induced
production of CSF by human bone marrow (BM) cells in long- term culture or
human fibroblasts. We showed that IL-6 does not induce CSF production by
these cells. Neither addition of exogenous IL-6 nor neutralization of
endogenous production of IL-6 by an anti-IL-6 monoclonal antibody (MoAb)
diminished the IL-1-induced colony- stimulating activity (CSA), indicating
that IL-6 did not act synergistically with IL-1. Finally, IL-6 did not
influence the kinetics of IL-1-induced CSA production by human fibroblasts.
We conclude that IL-6, either alone or in combination with IL-1, does not
induce CSF production by human BM stromal cells or fibroblasts.
Volume 74,
Issue 8,
pp. 2619-2623,
12/01/1989
Copyright © 1989 by The American Society of Hematology
