Identification through chemical cross-linking of distinct granulocyte-
macrophage colony-stimulating factor and interleukin-3 receptors on myeloid
leukemic cells, KG-1
T Gesner, RA Mufson, KJ Turner and SC Clark
Genetics Institute, Cambridge, MA.
Granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin-3
(IL-3) each bind specifically to a small number of high- affinity receptors
present on the surface of the cells of the acute myelogenous leukemia line,
KG-1. Through chemical cross-linking of IL-3 and GM-CSF to KG-1 cells, we
identified distinct binding proteins for each of these cytokines with
approximate molecular masses of 69 and 93 Kd, respectively. Although these
two binding proteins are distinct, GM- CSF and IL-3 compete with each other
for binding to KG-1 cells. Other cell lines, which express receptors for
either factor but not for both do not display this cross-competition for
binding with IL-3 and GM-CSF. These findings imply that distinct IL-3 and
GM-CSF binding proteins are expressed on the cell surface and that an
association exists between these proteins on KG-1 cells.
Volume 74,
Issue 8,
pp. 2652-2656,
12/01/1989
Copyright © 1989 by The American Society of Hematology
