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Tissue factor and factor VII messenger RNAs in human alveolar macrophages:
effects of breathing ozone
MP McGee, R Devlin, G Saluta and H Koren
Department of Medicine, Bowman Gray School of Medicine, Winston-Salem, NC
27103.
This study was performed to determine if genes for tissue factor and factor
VII proteins are expressed and regulated in vivo in lung macrophages during
inflammation. Human alveolar macrophages and alveolar fluids were obtained
18 hours after healthy male adults were exposed, for 2 hours during
intermittent exercise, to either air or air with 0.4 ppm ozone, added as a
model toxic respiratory agent. Messenger RNA (mRNA) for both tissue factor
and factor VII were demonstrated in macrophages isolated after subjects
were exposed to unpolluted control air. With the same subjects examined
after breathing ozone, the following changes were observed: tissue factor
mRNA concentration in macrophages increased 2.6 +/- 0.47-fold. Factor VII
mRNA concentration was reduced 0.64 +/- 0.24-fold. Total numbers of
macrophages recovered did not change significantly. Ratios of
nuclear:cytoplasmic areas of cytocentrifuged macrophages were augmented by
24.8% +/- 3%, giving morphometric evidence that immature cell forms
increased in the population. In the lavage, tissue factor activity was
increased 2.1 +/- 0.3-fold, while amounts of lipid phosphorous, which
estimate total membrane lipids, and estimated volumes of alveolar fluid
were not significantly changed. Factor VII activity and fibrinopeptide A
levels in lavage were increased approximately twofold. These results using
rapidly isolated, noncultured cells indicate that tissue factor and factor
VII mRNA are synthesized in the alveolar macrophage population in vivo. In
addition, evidence was found that as a result of breathing ozone, a shift
in alveolar macrophage maturity occurred in association with tissue factor
mRNA, tissue factor activity, and factor VII activity increases, and with
formation of fibrinopeptide A in alveolar fluids.
Volume 75,
Issue 1,
pp. 122-127,
01/01/1990
Copyright © 1990 by The American Society of Hematology
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