|
|
 Previous Article | Table of Contents | Next Article 
Detection of interleukin-5 messenger RNA in Reed-Sternberg cells of
Hodgkin's disease with eosinophilia
M Samoszuk and L Nansen
Pathology Department, University of California, Irvine 92717.
Hodgkin's disease of nodular sclerosis and mixed cellularity subtypes is
frequently associated with eosinophilia. To determine if interleukin- 5
(IL-5) is implicated in producing the eosinophilia, we performed in situ
hybridization studies on cytopreparations of 16 cases of Hodgkin's disease
with eosinophilia as well as cells from various controls. A
single-stranded, anti-sense complementary DNA (cDNA) probe coding for a
portion of the human IL-5 molecule was tail-labeled with digoxigenin -
11-dUTP using terminal transferase, and then hybridized to messenger RNA
(mRNA) within cells. An alkaline-phosphatase-conjugated antibody directed
to digoxigenin was used with a chromogenic substrate to detect hybridized
probe within cells. In all seven cases of nodular sclerosis subtype and
nine cases of mixed cellularity subtype with eosinophilia, there was strong
hybridization signal localizable to the cytoplasm of morphologically
identifiable Reed-Sternberg cells and variants. Similar activity was
detected only in rare cells from three normal spleens, and was undetectable
in two cell lines used as a negative control and in one case of Hodgkin's
disease without eosinophilia. Pretreatment of the cytopreparations with the
RNase inhibitor diethylpyrocarbonate greatly increased the hybridization
signal. Based on this controlled study, we conclude that mRNA coding for
IL-5 is expressed in Reed-Sternberg cells and variants. This observation
may explain the eosinophilia associated with Hodgkin's disease and provide
insight into the origin of the Reed- Sternberg cell.
Volume 75,
Issue 1,
pp. 13-16,
01/01/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Y. Ma, L. Visser, H. Roelofsen, M. de Vries, A. Diepstra, G. van Imhoff, T. van der Wal, M. Luinge, G. Alvarez-Llamas, H. Vos, et al.
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes
Blood,
February 15, 2008;
111(4):
2339 - 2346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Re, R. Kuppers, and V. Diehl
Molecular Pathogenesis of Hodgkin's Lymphoma
J. Clin. Oncol.,
September 10, 2005;
23(26):
6379 - 6386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Tancrede-Bohin, M. A. Ionescu, P. de La Salmoniere, A. Dupuy, J. Rivet, M. Rybojad, L. Dubertret, H. Bachelez, C. Lebbe, and P. Morel
Prognostic Value of Blood Eosinophilia in Primary Cutaneous T-Cell Lymphomas
Arch Dermatol,
September 1, 2004;
140(9):
1057 - 1061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Matsumoto, M. Terakawa, K. Miura, S. Fukuda, T. Nakajima, and H. Saito
Extremely Rapid and Intense Induction of Apoptosis in Human Eosinophils by Anti-CD30 Antibody Treatment In Vitro
J. Immunol.,
February 15, 2004;
172(4):
2186 - 2193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Portis, P. Dyck, and R. Longnecker
Epstein-Barr Virus (EBV) LMP2A induces alterations in gene transcription similar to those observed in Reed-Sternberg cells of Hodgkin lymphoma
Blood,
December 1, 2003;
102(12):
4166 - 4178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Persson-Dajotoy, P. Andersson, A. Bjartell, J. Calafat, and A. Egesten
Expression and Production of the CXC Chemokine Growth-Related Oncogene-{alpha} by Human Eosinophils
J. Immunol.,
May 15, 2003;
170(10):
5309 - 5316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. F. Skinnider and T. W. Mak
The role of cytokines in classical Hodgkin lymphoma
Blood,
May 29, 2002;
99(12):
4283 - 4297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. F. Skinnider, A. J. Elia, R. D. Gascoyne, B. Patterson, L. Trumper, U. Kapp, and T. W. Mak
Signal transducer and activator of transcription 6 is frequently activated in Hodgkin and Reed-Sternberg cells of Hodgkin lymphoma
Blood,
January 15, 2002;
99(2):
618 - 626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. F. Skinnider, A. J. Elia, R. D. Gascoyne, L. H. Trumper, F. von Bonin, U. Kapp, B. Patterson, B. E. Snow, and T. W. Mak
Interleukin 13 and interleukin 13 receptor are frequently expressed by Hodgkin and Reed-Sternberg cells of Hodgkin lymphoma
Blood,
January 1, 2001;
97(1):
250 - 255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. von Wasielewski, S. Seth, J. Franklin, R. Fischer, K. Hubner, M. L. Hansmann, V. Diehl, and A. Georgii
Tissue eosinophilia correlates strongly with poor prognosis in nodular sclerosing Hodgkin's disease, allowing for known prognostic factors
Blood,
February 15, 2000;
95(4):
1207 - 1213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Kapp, W.-C. Yeh, B. Patterson, A. J. Elia, D. Kagi, A. Ho, A. Hessel, M. Tipsword, A. Williams, C. Mirtsos, et al.
Interleukin 13 Is Secreted by and Stimulates the Growth of Hodgkin and Reed-Sternberg Cells
J. Exp. Med.,
June 21, 1999;
189(12):
1939 - 1946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Aster
Lymphocyte-Predominant Hodgkin's Disease: How Little Therapy Is Enough?
J. Clin. Oncol.,
March 1, 1999;
17(3):
744 - 744.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Matsumoto, H. Matsumoto, M. Seki, M. Hata, Y. Asano, S. Kanegasaki, R. L. Stevens, and M. Hirashima
Human Ecalectin, a Variant of Human Galectin-9, Is a Novel Eosinophil Chemoattractant Produced by T Lymphocytes
J. Biol. Chem.,
July 3, 1998;
273(27):
16976 - 16984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Gorlin and J. A. Ferry
Case 26-1996- A Seven-Year-Old-Boy with Fever, Lymphadenopathy, Hepatosplenomegaly, and Prominent Eosinophilia
N. Engl. J. Med.,
August 22, 1996;
335(8):
577 - 584.
[Full Text]
[PDF]
|
|
|
|
|
