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Granulocyte colony-stimulating factor stimulates human mature neutrophilic
granulocytes to produce interferon-alpha
N Shirafuji, S Matsuda, H Ogura, K Tani, H Kodo, K Ozawa, S Nagata, S Asano and F Takaku
Department of Hematology-Oncology, University of Tokyo, Japan.
Granulocyte colony-stimulating factor (G-CSF) is a glycoprotein hormone
that specifically stimulates both production and functional activation of
neutrophils, while interferon-alpha (IFN-alpha) is known to suppress
myelopoiesis, including neutrophil production in vivo and in vitro. On a
possibility that IFN-alpha may operate as one of the inhibitory feedback
factors in neutropoiesis, we examined whether neutrophils produce IFN-alpha
in response to G-CSF. Northern blot analysis showed that messenger RNA
(mRNA) for human IFN-alpha 1 became detectable time- dependently in highly
purified human neutrophils incubated with purified recombinant human G-CSF
(rhG-CSF). But such transcription was not observed either in neutrophils
incubated with other neutrophil activators, such as
formyl-methionyl-leucyl-phenylalanine (fMLP) or lipopolysaccharides (LPS),
or in blood mononuclear cells incubated with rhG-CSF. In addition,
radioimmunoassay for human IFN-alpha showed that its levels in culture
medium of the rhG-CSF-treated neutrophils rose markedly (up to
approximately 100 IU/mL/1 x 10(7) cells) in a time- dependent way, compared
with those of nonstimulated neutrophils. These findings suggest that the
G-CSF/IFN-alpha system may participate in the feedback regulatory loop of
neutropoiesis.
Volume 75,
Issue 1,
pp. 17-19,
01/01/1990
Copyright © 1990 by The American Society of Hematology
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