Effects of irradiation of recipient mice on the behavior and leukemogenic
potential of factor-dependent hematopoietic cell lines
U Duhrsen and D Metcalf
Cancer Research Unit, Walter and Eliza Hall Institute of Medical Research,
Royal Melbourne Hospital, Victoria, Australia.
After intravenous (IV) injection with factor-dependent FDC-P1 cells,
irradiated DBA/2 and BALB/c mice developed transplantable leukemias owing
to neoplastic transformation of the injected cells in vivo. Increasing the
radiation dose shortened the preleukemic latent period, and in female mice
the frequency of leukemia development was higher and the latent period
shorter than in male mice. In the preleukemic period, the injected FDC-P1
cells rapidly increased in number in hematopoietic organs of irradiated
animals, reaching peak levels 3 to 5 weeks after injection;
factor-independent transformed cells were not detected before day 45. In
unirradiated animals, these events were delayed by several weeks, and
long-term survivors did not harbor detectable FDC-P1 cells. FDC-P1 cells
sampled from preleukemic mice frequently showed atypical colony formation
and reduced cloning efficiency in vitro, suggesting the occurrence of a
distinct preleukemic change. U16.6 cells produced leukemia only in
irradiated recipients, and the leukemic cells usually remained factor
dependent. The two contrasting models should be of value in further
analyzing the mechanisms underlying radiation- induced leukemias.
Volume 75,
Issue 1,
pp. 190-197,
01/01/1990
Copyright © 1990 by The American Society of Hematology
