|
|
 Previous Article | Table of Contents | Next Article 
Biallelic neutrophil Na-antigen system is associated with a polymorphism on
the phospho-inositol-linked Fc gamma receptor III (CD16)
TW Huizinga, M Kleijer, PA Tetteroo, D Roos and AE von dem Borne
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service,
Amsterdam.
Neutrophils express two distinct types of receptor for the Fc region of
IgG, FcRII and FcRIII, in amounts of 10,000 to 20,000 FcRII (40 Kd) and
100,000 to 200,000 FcRIII (50 to 80 Kd) per neutrophil. We showed that the
FcRIII exhibits genetically determined heterogeneity, detectable by
differences in electrophoretic mobility with sodium dodecyl sulfate (SDS)
as well as by reaction with antibodies against the biallelic
neutrophil-specific antigen system NA. FcRIII was precipitated with an
FcRIII-specific monoclonal antibody (MoAb) from the neutrophils of 35
donors. NA1NA1 donors expressed an FcRIII with a molecular weight (mol wt)
of 50 to 65 Kd, NA1NA2 donors expressed an FcRIII with a mol wt of 50 to 80
Kd, and NA2NA2 donors expressed an FcRIII with a mol wt of 65 to 80 Kd.
Statistical analysis showed that the electrophoretic heterogeneity
corresponds with the NA polymorphism (k = 1). Sequential
immunoprecipitation with a MoAb against NA1 and a MoAb against anti-
FcRIII, followed by SDS-polyacrylamide gel electrophoresis (PAGE), showed
that NA1-FcRIII is distinct from NA2-FcRIII. Moreover, immunoprecipitation
with a MoAb against NA1 yielded a protein of 50 to 65 Kd, and
immunoprecipitation with human anti-NA2 sera or an MoAb against NA2 yielded
a protein of 65 to 80 Kd. Preincubation of NA1NA2 neutrophils with F(ab')2
fragments of an MoAb against anti-NA1 reduced binding of IgG dimers to
these cells with about 50%, whereas it completely prevented binding of the
dimers to NA1NA1 neutrophils. Inhibition experiments with the MoAb against
NA2 yielded the same results for NA1NA2 cells, whereas binding of IgG
dimers to NA2NA2 cells was completely prevented. Thus, the products of both
NA alleles bind IgG. Immunoprecipitation from the medium of neutrophils
either stimulated with formyl- methionyl-leucyl-phenylalanine (FMLP) or
treated with glycosyl-phosphatidyl-inositol-specific phospholipase C (GPI-
PLC) showed that both the NA1-FcRIII and the NA2-FcRIII are released from
the cell surface, indicating that both forms of FcRIII have some structural
features in common. Deglycosylation of FcRIII from homozygous donors
yielded material that showed several bands on SDS- PAGE. GPI-PLC treatment
of neutrophils indicated that all of this material is phosphatidyl-inositol
linked.
Volume 75,
Issue 1,
pp. 213-217,
01/01/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Ota, M. Aoki-Ota, B. H. Duong, and D. Nemazee
Suppression of IgE B Cells and IgE Binding to Fc{epsilon}RI by Gene Therapy with Single-Chain Anti-IgE
J. Immunol.,
June 15, 2009;
182(12):
8110 - 8117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. B. Breunis, E. van Mirre, M. Bruin, J. Geissler, M. de Boer, M. Peters, D. Roos, M. de Haas, H. R. Koene, and T. W. Kuijpers
Copy number variation of the activating FCGR2C gene predisposes to idiopathic thrombocytopenic purpura
Blood,
February 1, 2008;
111(3):
1029 - 1038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Rocha, R. F. Franco, R. Porcher, H. Bittencourt, W. A. Silva Jr, A. Latouche, A. Devergie, H. Esperou, P. Ribaud, G. Socie, et al.
Host defense and inflammatory gene polymorphisms are associated with outcomes after HLA-identical sibling bone marrow transplantation
Blood,
December 1, 2002;
100(12):
3908 - 3918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Durand, J.-O. Pers, Y. Renaudineau, A. Saraux, P. Youinou, and C. Jamin
Differential effects of anti-Fc{gamma}RIIIb autoantibodies on polymorphonuclear neutrophil apoptosis and function
J. Leukoc. Biol.,
February 1, 2001;
69(2):
233 - 240.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. C. A. A. van Schie and M. E. Wilson
Evaluation of Human Fcgamma RIIA (CD32) and Fcgamma RIIIB (CD16) Polymorphisms in Caucasians and African-Americans Using Salivary DNA
Clin. Vaccine Immunol.,
July 1, 2000;
7(4):
676 - 681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C.A. Bruin, A. E.G.K. von dem Borne, R. Y.J. Tamminga, M. Kleijer, L. Buddelmeijer, and M. de Haas
Neutrophil Antibody Specificity in Different Types of Childhood Autoimmune Neutropenia
Blood,
September 1, 1999;
94(5):
1797 - 1802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bux, K. Kissel, C. Hofmann, and S. Santoso
The Use of Allele-Specific Recombinant Fcgamma Receptor IIIb Antigens for the Detection of Granulocyte Antibodies
Blood,
January 1, 1999;
93(1):
357 - 362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bux, E.-L. Stein, P. Bierling, P. Fromont, M. Clay, D. Stroncek, and S. Santoso
Characterization of a New Alloantigen (SH) on the Human Neutrophil Fcgamma receptor IIIb
Blood,
February 1, 1997;
89(3):
1027 - 1034.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
