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Regulation of megakaryocyte ploidy in vivo in the rat
DJ Kuter and RD Rosenberg
Department of Biology, Massachusetts Institute of Technology, Cambridge
02139.
The relationship between the bone marrow (BM) megakaryocyte and the
circulating platelet was explored. Incremental changes in platelet count
were made in rats by infusion of antiplatelet antibody or by platelet
transfusion, and the response of megakaryocytes was measured by flow
cytometry. Proportional changes in megakaryocyte ploidy were demonstrated:
As the platelet count declined, ploidy increased; as the platelet count
increased, ploidy decreased. Even moderate degrees of thrombocytopenia and
thrombocytosis (48% and 177% of the normal platelet count) were associated
with changes in ploidy. These changes were not the results of the technique
used to alter the platelet count because reinfusion of platelets after 3
hours of thrombocytopenia prevented any ploidy change. These studies proved
that the circulating platelet and the megakaryocyte constitute a classic
feedback loop whose activity can be measured by the degree of ploidization
of the megakaryocyte. The minimal duration of thrombocytopenia necessary to
promote megakaryocyte ploidy changes was approximately 10 hours. Using a BM
culture assay, we identified a plasma factor which induced alterations in
megakaryocyte ploidy and whose level is inversely proportional to the
platelet count.
Volume 75,
Issue 1,
pp. 74-81,
01/01/1990
Copyright © 1990 by The American Society of Hematology
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