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 Previous Article | Table of Contents | Next Article 
Human P40 T-cell growth factor (interleukin-9) supports erythroid colony
formation
RE Donahue, YC Yang and SC Clark
Genetics Institute Inc, Cambridge, MA.
Because human P40 T-cell growth factor, tentatively designated
interleukin-9 (IL-9), was isolated through its ability to stimulate a human
IL-3-dependent leukemic cell line (M-O7E), we tested the ability of IL-9 to
support the growth and differentiation of normal hematopoietic progenitor
cells from peripheral blood and bone marrow. Although the M-O7E cell line
was derived from a patient with megakaryoblastic leukemia, IL-9 has not
proved to be a growth or maturation factor for megakaryocytes, but instead
has proved to be effective in supporting the development of erythroid
bursts (BFU-E) in cultures supplemented with erythropoietin. Using highly
purified progenitors from peripheral blood, IL-3 showed a BFU-E plating
efficiency of 46% compared with 20% for IL-9. Because of the purity of
these cell preparations and the low cell density in culture, IL-9 is likely
to interact directly with erythroid progenitors. Analysis of mixing
experiments and of the morphology of the BFU-E in culture indicated that
IL-9 interacts preferentially with a relatively early population of
IL-3-responsive BFU-E. In cultures of human bone marrow or cord blood, IL-9
selectively supported erythroid colony formation, while IL-3 and
granulocyte/macrophage colony-stimulating factor additionally yielded
granulocyte/macrophage colonies. Therefore, IL-9 represents a new T
cell-derived cytokine with the potential for selectively stimulating
erythroid development in the hematopoietic system.
Volume 75,
Issue 12,
pp. 2271-2275,
06/15/1990
Copyright © 1990 by The American Society of Hematology
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