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Effects of interleukin-3 and interleukin-4 on the development of
"connective tissue-type" mast cells: interleukin-3 supports their survival
and interleukin-4 triggers and supports their proliferation synergistically
with interleukin-3
K Tsuji, T Nakahata, M Takagi, T Kobayashi, A Ishiguro, T Kikuchi, K Naganuma, K Koike, A Miyajima and K Arai
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,
Japan.
We examined the effects of interleukin-3 (IL-3) and interleukin-4 (IL- 4)
on connective tissue-type mast cells (CTMC) purified from murine peritoneal
cells. Although both factors failed to induce extensive proliferation of
CTMC, they stimulated CTMC proliferation synergistically in a
dose-dependent manner. Pretreatment of CTMC with IL-3 and/or IL-4 indicated
that the sustained presence of both factors was required for the
development of type 1 mast cell colonies. The delayed addition of IL-3 to
cultures of purified CTMC with IL-4 induced no colony formation, while the
delayed addition of IL-4 to cultures with IL-3, even on day 28 of culture,
induced type 1 colony formation. In replating type 1 colonies induced by
IL-3 and IL-4 to secondary cultures with IL-3 alone, few secondary colonies
developed. However, the delayed addition of IL-4 to the secondary culture
induced many type 1 colonies. The purified CTMC cultured with IL-3 retained
the morphological and cytochemical characteristics of CTMC, as well as
proliferative ability. These observations indicate that IL-3 supports the
survival of CTMC in methylcellulose culture and that IL-4 triggers and
supports CTMC proliferation synergistically with IL-3. The serum- free
culture of purified CTMC and the culture of single CTMC demonstrated that
the synergistic effect of IL-3 and IL-4 on colony growth and the surviving
effect of IL-3 on CTMC require no influence from accessory cells or other
humoral factors.
Volume 75,
Issue 2,
pp. 421-427,
01/15/1990
Copyright © 1990 by The American Society of Hematology
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