|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
K Sugiura, S Ikehara, N Gengozian, M Inaba, EE Sardina, H Ogata, SM Seong and RA Good
Department of Pediatrics, All Children's Hospital, University of South
Florida, St Petersburg 33701.
Natural suppressor (NS) activity, the capacity of unprimed cells to
suppress immunologic responses, is present in mouse, rabbit, and human bone
marrow (BM). In this study we characterize NS activity in bone marrow cells
of the rhesus monkey. Greatest NS activity was found in low-density cells
(1.0600 to 1.0655 g/mL) obtained by density centrifugation on a
discontinuous Percoll gradient. NS activity was further enriched when cells
were separated by affinity for wheat germ agglutinin (WGA). Cells with high
affinity to WGA demonstrated potent NS activity, whereas cells with low
affinity to WGA had no NS activity. A significant relationship between NS
activity and hematopoietic activity was demonstrated using in vitro assays
of colony formation (CFU-GM and CFU-MIX). NS activity was not affected by
treatment with monoclonal antibodies (MoAbs) to human Fc gamma receptors
(Leu, 11a,b,c) or treatment with MoAbs to monkey natural killer cells.
These findings extend our prior observations by showing that cells with NS
activity, which apparently have WGA receptors, are present not only in
murine BM but also in monkey bone marrow, and suggest that such cells may
be involved in immunoregulation by primitive cells of BM.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1990 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
