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Establishment and characterization of human B-cell lymphoma cell lines
using B-cell growth factor
RJ Ford, A Goodacre, I Ramirez, SR Mehta and F Cabanillas
Department of Molecular Pathology, M.D. Anderson Cancer Center, University
of Texas, Houston 77030.
B-cell non-Hodgkin's lymphomas (NHL-B) have been difficult to establish in
long-term cell culture using standard techniques. We report the
establishment of five representative cell lines from high grade NHL-B using
B-cell growth factor (BCGF). The five NHL-B cell lines display the
morphologic, immunophenotypic, genotypic, and biologic characteristics of
the lymphoma cells present in the original diagnostic specimen. The cell
lines showed at least a sevenfold dose- dependent increase in proliferation
in vitro over background in the presence of BCGF. Other putative B-cell
growth-stimulating cytokines showed no significant proliferative activity
or were inhibitory in some cases. NHL-B cell lines secreted growth
factor(s) into culture supernatants that mediated at least a fivefold
dose-dependent increase in cell proliferation in autochthonous lymphoma
cells and a 10-fold or greater stimulation in growth factor-dependent
normal B cell lines in vitro. The cell lines show monoclonal rearrangements
of IgH genes and nonrandom chromosomal abnormalities characteristic of
NHL-B, while the expression of Epstein-Barr virus associated antigen
(EBNA-I) is present in two of the five cell lines. The studies show that
lineage-specific growth factors may be used to establish neoplastic B cell
lines in vitro, which are important experimental systems for cellular and
molecular studies in the NHL-B.
Volume 75,
Issue 6,
pp. 1311-1318,
03/15/1990
Copyright © 1990 by The American Society of Hematology
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