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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Offit, K. Articles by Chaganti, R. S. Search for Related Content PubMed PubMed Citation Articles by Offit, K. Articles by Chaganti, R. S. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Cytogenetic analysis of chimerism and leukemia relapse in chronic myelogenous leukemia patients after T cell-depleted bone marrow transplantation K Offit, JP Burns, I Cunningham, SC Jhanwar, P Black, NA Kernan, RJ O'Reilly and RS Chaganti Laboratory of Cancer Genetics and Cytogenetics, Sloan Kettering Institute, New York, NY 10021. Serial cytogenetic studies were performed on 64 patients with chronic myelogenous leukemia (CML) after T cell-depleted allogeneic bone marrow transplantation (BMT). Forty patients with CML in chronic phase (CP) received cytoreduction followed by BMT with HLA-matched T cell-depleted allogeneic marrow. The remaining 24 patients were transplanted in second chronic, accelerated, or blastic phase, or received T cell- depleted grafts with a dose of T cells added back. The Y chromosome and autosomal heteromorphisms were used to distinguish between donor and host cells. Mixed hematopoietic chimerism (presence of donor and host cells) was identified in 90% of patients in first CP. The Philadelphia (Ph) chromosome reappeared in 16 of the 40 first CP CML patients. As expected, patients who had detectable Ph chromosome positive cells at any time during the posttransplant period had a high likelihood of subsequent clinical relapse. Transient disappearance of the Ph positive clone was rarely observed, and was followed by reappearance of the Ph chromosome or clinical relapse. A subset of engrafted patients with greater than 25% host cells within 3 months post-BMT had a significantly shorter survival time free of cytogenetic or clinical relapse compared with other patients. In patients who had received donor T cells added to the T cell-depleted graft, there was a higher proportion of complete chimerism. Clonal progression of Ph positive as well as negative cells was observed and may be the result of radiation induced breakage. Serial cytogenetic studies of patients post-BMT can provide useful information regarding the biologic and clinical behavior of CML. Volume 75, Issue 6, pp. 1346-1355, 03/15/1990 Copyright © 1990 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: V. T. Ho and R. J. Soiffer The history and future of T-cell depletion as graft-versus-host disease prophylaxis for allogeneic hematopoietic stem cell transplantation Blood, December 1, 2001; 98(12): 3192 - 3204. [Full Text] [PDF] C.-M. Seong, S. Giralt, H. Kantarjian, J. Xu, J. Swantkowski, K. Hayes, A. B. Glassman, I. Khouri, M. Korbling, P. Thall, et al. Early Detection of Relapse by Hypermetaphase Fluorescence In Situ Hybridization After Allogeneic Bone Marrow Transplantation for Chronic Myeloid Leukemia J. Clin. Oncol., May 9, 2000; 18(9): 1831 - 1836. [Abstract] [Full Text] [PDF] A Toren, G Rechavi, and A Nagler Minimal residual disease post-bone marrow transplantation for hemato- oncological diseases Stem Cells, May 1, 1996; 14(3): 300 - 311. [Abstract]

	Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020