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Cytolytic function of clonable T cells after human bone marrow
transplantation
A Velardi, P Varese, CE Grossi, N Albi, C Dembech, A Terenzi, L Moretta, F Grignani, MF Martelli and MC Mingari
Department of Internal Medicine, University of Perugia, Italy.
We evaluated T-cell mediated lymphokine activated killer (LAK) function
during the late (greater than 5 months) reconstitution phase after T
cell-depleted allogeneic bone marrow transplantation (BMT) for hematologic
malignancy. Since LAK cells are sustained by interleukin-2 (IL-2), we also
investigated the ability of post-BMT T cells to produce IL-2. These
functions were investigated at the clonal level. More than 200 T-cell
clones from six long-term BMT recipients were generated and compared with
60 T-cell clones derived from two normal controls. Almost all the CD8+
clonal cultures from BMT recipients expressed cytolytic activity in a
lectin-dependent cellular cytoxicity assay. Interestingly, a higher
proportion of BMT recipient-derived cytolytic clones were able to mediate
LAK activity in comparison with control clones (28% versus 4%, P less than
.05). However, T-cell clones from BMT recipients, as opposed to control
clones, were largely incapable of producing IL-2. Given the high
proportions of post-BMT circulating CD8+ T cells, it appears that, in
long-term BMT recipients, the precursors of nonspecific LAK effectors are
present at above normal levels. However, their function may be defective in
vivo due to poor IL-2 production.
Volume 75,
Issue 6,
pp. 1364-1369,
03/15/1990
Copyright © 1990 by The American Society of Hematology
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