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Antithrombotic effect of recombinant human thrombomodulin on thrombin-
induced thromboembolism in mice
K Gomi, M Zushi, G Honda, S Kawahara, O Matsuzaki, T Kanabayashi, S Yamamoto, I Maruyama and K Suzuki
Bio-Science Laboratory, Asahi Chemical Industry, Fuji City, Japan.
Antithrombotic effect of recombinant human thrombomodulin in mice, both in
vitro and in vivo, was studied. The soluble recombinant human
thrombomodulin was expressed in Chinese hamster ovary cells and purified
from the conditioned medium by a modification of the conventional method.
Recombinant thrombomodulin prolonged thrombin clotting time for mouse
plasma in a dose-dependent manner. Thrombin was injected into the lateral
tail vein of mice and caused acute thromboembolism. All mice injected with
thrombin died of thromboembolism; however, preinjection with recombinant
human thrombomodulin neutralized the lethal effect of thrombin in a
concentration-dependent manner. Histologic examination showed that fibrin
deposits were found in all large and small arteries in the lung from mice
injected with thrombin; however, fibrin deposits were not detected in any
large arteries from the mouse preinjected with thrombomodulin.
Volume 75,
Issue 7,
pp. 1396-1399,
04/01/1990
Copyright © 1990 by The American Society of Hematology
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