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Characterization and fibrinolytic properties of human omental tissue
mesothelial cells. Comparison with endothelial cells
VW van Hinsbergh, T Kooistra, MA Scheffer, J Hajo van Bockel and GN van Muijen
Gaubius Institute TNO, Leiden, The Netherlands.
It has been reported that omental fat tissue is a good source of human
microvascular endothelial cells. By characterization we demonstrate that
the epitheloid cells isolated from omental tissue are not endothelial
cells, but mesothelial cells. They contain abundant cytokeratins 8 and 18,
which are absent in endothelial cells, and vimentin. No staining with the
endothelial-specific antibodies EN-4 and PAL-E is observed. A faint and
diffuse staining of von Willebrand factor (vWF) is seen in mesothelial
cells, whereas microvascular endothelial cells from subcutaneous fat
display vWF in distinct granular structures. Human peritoneal mesothelium
produces plasminogen activator-dependent fibrinolytic activity, which is
essential in the resolution of fibrous exudates and may therefore be
important in preventing the formation of fibrous peritoneal adhesions. This
fibrinolytic activity is plasminogen activator-dependent, but has not been
fully characterized. We report here that human omental tissue mesothelial
cells in vitro produce large amounts of tissue-type plasminogen activator
(t-PA), together with type 1 and 2 plasminogen activator inhibitor (PAI-1
and PAI-2). PAI-1 is predominantly secreted into the culture medium,
whereas the major part of PAI-2 is found in the cells. No urokinase-type
plasminogen activator is detected. On stimulation with the inflammatory
mediator tumor necrosis factor (TNF), at least a threefold decrease in t-PA
antigen is observed, together with an increase in both PAI-1 and PAI-2. TNF
also induces a marked change in cell shape. Whereas TNF and bacterial
lipopolysaccharide (LPS) have similar effects on the production of PA
inhibitor by human endothelial cells, LPS has no or only a relatively small
effect on the fibrinolytic properties of mesothelial cells. The decreased
fibrinolytic activity induced by the cytokine TNF may impair the natural
dissolution of fibrin deposits at the peritoneum in the presence of an
inflammatory reaction.
Volume 75,
Issue 7,
pp. 1490-1497,
04/01/1990
Copyright © 1990 by The American Society of Hematology
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