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Phorbol 12-myristate 13-acetate-induced development of functionally active
mast cells in W/Wv but not Sl/Sld genetically mast cell- deficient mice
JR Gordon and SJ Galli
Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
The normal skin and other tissues of adult genetically mast cell- deficient
WBB6F1-W/Wv or WCB6F1-Sl/Sld mice contain less than 1.0% the number of mast
cells present in the corresponding tissues of the congenic normal (+/+)
mice. We previously reported that mature dermal mast cells developed
locally in the skin of W/Wv, but not Sl/Sld, mice at sites of chronic
idiopathic dermatitis. We now report that the repeated application of
phorbol 12-myristate 13-acetate (PMA) to the ear skin of either W/Wv or +/+
mice induces both dermatitis and a striking and dose-dependent increase in
the number of dermal mast cells. The number of dermal mast cells at sites
treated for 6 weeks with 5 micrograms PMA, three times per week, was 39 +/-
7/mm2 and 305 +/- 34/mm2 for W/Wv and +/+ mice, respectively; the
corresponding values for vehicle-treated skin were 1.5 +/- 1.0/mm2 and 145
+/- 8/mm2, respectively. The PMA-induced dermal mast cells in W/Wv mice
appeared mature by morphology, stained with the heparin-binding fluorescent
dye, berberine sulfate, and were competent to express IgE-dependent passive
cutaneous anaphylaxis responses. The development of mast cells was a local,
not systemic, effect of PMA treatment. PMA treatment also induced
dermatitis in both WCB6F1-Sl/Sld and +/+ mice, but was associated with
increased numbers of dermal mast cells only in the WCB6F1(-)+/+ mice. PMA
treatment had no detectable effect on the ability of bone marrow-derived
cultured mast cells to survive in the skin of Sl/Sld mice. These findings
establish a convenient model system for analyzing factors associated with
the development of endogenous populations of mast cells in genetically mast
cell-deficient W/Wv mice.
Volume 75,
Issue 8,
pp. 1637-1645,
04/15/1990
Copyright © 1990 by The American Society of Hematology
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