Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilhelm, O.
Right arrow Articles by Graeff, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilhelm, O.
Right arrow Articles by Graeff, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

Role of plasmin in the degradation of the stroma-derived fibrin in human ovarian carcinoma

O Wilhelm, R Hafter, A Henschen, M Schmitt and H Graeff

Frauenklinik der Technischen Universitat Munchen, FRG.

The aim of this study was to evaluate the type of enzymes involved in tumor-associated fibrinolysis of the stroma component fibrin in ovarian cancer patients. For this purpose, the high-molecular-mass fibrin degradation products (HMM-XDP) were isolated from malignant ascitic fluid by protamine sulfate precipitation and further purified by gel filtration and acid precipitation. After reduction with 2- mercaptoethanol, the peptide chain components were separated by reverse- phase high-performance liquid chromatography (RP-HPLC). The nature of these components was elucidated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid sequence analysis and compared with fibrin-derived fragments formed in vitro. The results indicate that plasmin is the essential protease involved in the degradation of the stroma-derived fibrin portion found in ovarian cancer ascites.

Volume 75, Issue 8, pp. 1673-1678, 04/15/1990
Copyright © 1990 by The American Society of Hematology


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CLIN APPL THROMB HEMOSTHome page
D. N. Contreras, E. Cobos, and C. Lox
Is the Circulating Urokinase Plasminogen Activator Upregulated by the Circulating p105 Fraction of the HER-2/neu Proto-oncogene in Patients with Cervical Cancer?
Clinical and Applied Thrombosis/Hemostasis, July 1, 2004; 10(3): 271 - 276.
[Abstract] [PDF]

Home page
 CLIN APPL THROMB HEMOSTHome page
E. Cobos, C. Jumper, and C. Lox
Pretreatment Determination of the Serum Urokinase Plasminogen Activator and its Soluble Receptor in Advanced Small-Cell Lung Cancer or Non-Small-Cell Lung Cancer
Clinical and Applied Thrombosis/Hemostasis, July 1, 2003; 9(3): 241 - 246.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
L. Goretzki, C. R. Lombardo, and W. B. Stallcup
Binding of the NG2 Proteoglycan to Kringle Domains Modulates the Functional Properties of Angiostatin and Plasmin(ogen)
J. Biol. Chem., September 8, 2000; 275(37): 28625 - 28633.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 1990 by American Society of Hematology         Online ISSN: 1528-0020