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Regulation of human eosinophil precursor production by cytokines: a
comparison of recombinant human interleukin-1 (rhIL-1), rhIL-3, rhIL-5,
rhIL-6, and rh granulocyte-macrophage colony-stimulating factor
EJ Clutterbuck and CJ Sanderson
National Institute for Medical Research, Ridgeway, London, UK.
The effect of a panel of recombinant human (rh) cytokines on the generation
of human eosinophil precursors was assessed using a two-step culture
technique. Normal human bone marrow was preincubated with different
cytokine combinations in liquid culture before assessment of the number of
eosinophil progenitors, which give rise to eosinophil colony-forming units
(CFU-Eo) on secondary semi-solid culture with either interleukin-5 (IL-5),
IL-3, or granulocyte-macrophage colony- stimulating factor. rhIL-3 or
rhGM-CSF, but not rhIL-5, increased the number of CFU-Eo. CFU-Eo production
by rhIL-3 or rhGMCSF was maximal after 7 days' preincubation. Neither
rhIL-1 or rhIL-6 acted on eosinophil precursors, either alone or in
combination with rhIL-5, rhIL- 3, or rhGM-CSF. A similar spectrum of
activity of the cytokines was demonstrated whether rhIL-5, rhIL-3, or
rhGM-CSF was used in the secondary cultures as the eosinophil CSF. However,
rhIL-3 induced relatively more rhIL-5-responsive CFU-Eo than
rhIL-3-responsive CFU-Eo, suggesting that rhIL-3 is pushing progenitors
into an rhIL-5-responsive compartment.
Volume 75,
Issue 9,
pp. 1774-1779,
05/01/1990
Copyright © 1990 by The American Society of Hematology
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