|
|
 Previous Article | Table of Contents | Next Article 
Localization of breakpoints by polymerase chain reactions in Burkitt's
lymphoma with 8;14 translocations
B Shiramizu and I Magrath
National Institutes of Health, Bethesda, MD 20892.
Translocations involving chromosomes 8 and 14 in Burkitt's lymphoma (BL)
often involve the switch mu (Smu) region on chromosome 14, which contains
multiple repeats. This has enabled us to use the polymerase chain reaction
(PCR) to detect breakpoints that involve this region on chromosome 14 and
the c-myc gene on chromosome 8. Using pairs of flanking primers, each pair
including one annealing to repeat sequences within the switch region and
one of three primers from the c-myc region (first intron, 3', or 5'
flanking sequence of the first exon of c-myc), we have been able to amplify
DNA fragments containing the corresponding breakpoint regions from
chromosome 14 in both cell lines and biopsied tumor samples. The definitive
demonstration of sequences from both chromosomes in these fragments
permitted the confirmation of the presence of a translocation. Because of
the sensitivity of PCR, we were able to localize breakpoints in samples
containing as few as 1 neoplastic cell in 10(8) cells. PCR provides a
valuable tool for the detection of 8;14 chromosomal translocations, which
should prove to be of value in diagnosis and molecular epidemiologic
studies, as well as providing a means of detecting minimal disease.
Volume 75,
Issue 9,
pp. 1848-1852,
05/01/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. A. Hill, S. S. Wang, J. R. Cerhan, S. Davis, W. Cozen, R. K. Severson, P. Hartge, S. Wacholder, M. Yeager, S. J. Chanock, et al.
Risk of non-Hodgkin lymphoma (NHL) in relation to germline variation in DNA repair and related genes
Blood,
November 1, 2006;
108(9):
3161 - 3167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. G. Janssen, J.-W. Vaandrager, T. Heuser, A. Jauch, P. M. Kluin, E. Geelen, P. L. Bergsagel, W. M. Kuehl, H. G. Drexler, T. Otsuki, et al.
Concurrent activation of a novel putative transforming gene, myeov, and cyclin D1 in a subset of multiple myeloma cell lines with t(11;14)(q13;q32)
Blood,
April 15, 2000;
95(8):
2691 - 2698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Basso, E. Frascella, L. Zanesco, and A. Rosolen
Improved Long-Distance Polymerase Chain Reaction for the Detection of t(8;14)(q24;q32) in Burkitt’s Lymphomas
Am. J. Pathol.,
November 1, 1999;
155(5):
1479 - 1485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Siebert, P. Matthiesen, S. Harder, Y. Zhang, A. Borowski, R. Zuhlke-Jenisch, S. Metzke, S. Joos, K. Weber-Matthiesen, W. Grote, et al.
Application of Interphase Fluorescence In Situ Hybridization for the Detection of the Burkitt Translocation t(8;14)(q24;q32) in B-Cell Lymphomas
Blood,
February 1, 1998;
91(3):
984 - 990.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
