Effects of phorbol esters on an interleukin-3-dependent cell line
JA McCubrey, LS Steelman, G Sandlin, RS Riddle and DK Ways
Department of Microbiology, East Carolina University School of Medicine,
Greenville, NC.
FDC-P1 is an interleukin-3 (IL-3)-dependent cell line that ceases to
proliferate in the absence of IL-3. We have isolated variant cell lines
from FDC-P1 that grow in response to phorbol myristate acetate (PMA). These
variant cell lines (FD/PMA) have maintained their PMA-dependency for over 1
year. Lymphokine gene expression, which would support growth, was not
detected in FD/PMA lines. FD/PMA lines had a different cell surface
phenotype than the parental line. Mac-1, Mac-2, and Mac-3 were readily
detected on the cell surface of FD/PMA lines; however, these antigens were
not detected on FDC-P1. Because protein kinase C (PKC) activation may
mediate PMA effects, we examined this kinase. PKC activity quantitated by
32P-incorporation into histone was increased in FDC-P1 as compared with
FD/PMA cultured in IL-3. Moreover, PKC activity was undetectable in FD/PMA
lines cultured in PMA. Using Western blotting, immunoreactive PKC was
readily detected in cytosolic and solubilized particulate fractions of
FDC-P1 cells, not but in FD/PMA cell extracts. Comparisons between the
parental and FD/PMA lines should provide insight into IL-3- and
PMA-mediated signal transduction.
Volume 76,
Issue 1,
pp. 63-72,
07/01/1990
Copyright © 1990 by The American Society of Hematology
