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Target cells for granulocyte colony-stimulating factor, interleukin-3, and
interleukin-5 in differentiation pathways of neutrophils and eosinophils
H Ema, T Suda, K Nagayoshi, Y Miura, CI Civin and H Nakauchi
Department of Medicine, Jichi Medical School, Tochigi-ken, Japan.
To study the relationship between hematopoietic factors and their
responsive hematopoietic progenitors in the differentiation process, both
purified factors and enriched progenitors are required. We isolated total
CD34+ cells, CD34+,CD33+ cells, and CD34+,CD33- cells individually from
normal human bone marrow cells by fluorescence- activated cell sorter
(FACS), and examined the effects of granulocyte colony-stimulating factor
(G-CSF), interleukin-3 (IL-3), and IL-5 on in vitro colony formation of
these cells. CD34+,CD33+ cells formed granulocyte colonies in the presence
of G-CSF. Both CD34+,CD33+ cells and CD34+,CD33- cells formed
granulocyte/macrophage colonies in the presence of IL-3. Eosinophil (Eo)
colonies were only formed by CD34+,CD33- cells in response to IL-3, but
scarcely formed by CD34+ cells in the presence of IL-5. We performed the
two-step cultures consisting of the primary liquid culture for 6 days and
the secondary methylcellulose culture, and serially examined changes in
phenotypes of ,he cells cultured in the primary culture. CD34-,CD33+ cells
derived from CD34+,CD33+ cells by preincubation with G-CSF or IL-3 formed
Eo colonies in the presence of IL-5 but not IL-3. CD34-,CD33+ cells derived
from CD34+,CD33- cells by preincubation with IL-3 also formed Eo colonies
by support of IL-5 as well as IL-3. Both CD34+ cells gradually lost the
CD34 antigen by day 6 of incubation with G-CSF or IL- 3. Loss of this
antigen was well-correlated with acquisition of susceptibility to IL-5. It
was concluded that G-CSF supported the neutrophil differentiation of
committed colony-forming cells, IL-3 supported that of both committed and
multipotent colony-forming cells. G-CSF and IL-3 also supported the early
stage of E. differentiation; IL- 5 supported the late stage of that.
Volume 76,
Issue 10,
pp. 1956-1961,
11/15/1990
Copyright © 1990 by The American Society of Hematology
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