Kidney cell lysates contain an activity that stimulates mature erythroid
burst-forming-unit (mBFU-E) proliferation
J Royet, G Mouchiroud, S Arnaud, T Oddos, S Galland and JP Blanchet
Centre de Genetique Moleculaire et Cellulaire, UMR CNRS n. 106, Universite
Claude Bernard, Villeurbanne, France.
This study reports the detection of an activity that stimulates the
development of a subclass of burst-forming unit-erythroid (BFU-E)
progenitors giving rise to small bursts in semi-solid cultures established
in the presence of saturating concentrations of erythropoietin. These
progenitors are considered to be mature BFU-E. The activity is found in
extracts from kidney cells and appears to be physiologically regulated as
it was respectively enhanced and decreased in kidneys from anemic and
polycythemic mice. The disappearance of activity in kidney-cell extracts
during long-term polycythemia correlated with an accumulation of mature
BFU-E in the spleen and bone marrow of polycythemic mice. Using specific
neutralizing antibodies and in vitro tests, we also show that this activity
is different from hemopoietins known to share burst promoting activity
(Interleukin-3 [IL- 3], granulocyte-macrophage colony-stimulating factor
[GM-CSF], Interleukin-4 [IL-4], erythropoietin [EPO], human interleukin for
DA cells [HILDA]) and that it can stimulate erythroid differentiation in
long term bone marrow cell cultures.
Volume 76,
Issue 10,
pp. 1965-1971,
11/15/1990
Copyright © 1990 by The American Society of Hematology
