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Cell membrane expression and functional role of the p75 subunit of
interleukin-2 receptor in lymphoproliferative disease of granular
lymphocytes
R Zambello, L Trentin, G Pizzolo, P Bulian, M Masciarelli, C Feruglio, C Agostini, R Raimondi, T Chisesi and G Semenzato
Department of Clinical Medicine, Padua University School of Medicine,
Italy.
The cell membrane expression and functional role of the interleukin-2
receptor (IL-2R) was analyzed in nine patients with lymphoproliferative
disease of granular lymphocytes (LDGL) using monoclonal antibodies (MoAbs)
specific for the p75 (TU27) and the p55 (anti-Tac) subunits of IL-2R. Four
patients were characterized by the proliferation of CD3+CD8+ granular
lymphocytes (GL) expressing the alpha/beta T-cell receptor (T alpha beta)
and one case by the proliferation of CD3+CD4- CD8- GL expressing the
gamma/delta T-cell receptor (T gamma delta); in four additional cases
proliferating cells were CD3 negative GL. Consistent with data observed on
normal GL, phenotypic analysis demonstrated that patients' GL lack the
expression of the p55 IL-2R, whereas the p75 subunit is constitutionally
expressed by expanding GL of both T-cell (either T alpha beta and T gamma
delta) and natural killer (NK) origin in variable proportions (11% to 94%
of cells). The analysis of the cytotoxic and proliferative activity
demonstrated that the anti-p55 MoAb failed to inhibit IL-2-mediated
activation, whereas a marked inhibition of both cytotoxicity and
proliferation were obtained using the anti-p75 chain specific MoAb. These
data indicate that the p75 chain of IL-2R is responsible for IL-2 signal
transduction in both CD3+ and CD3- LDGL patients' GL.
Volume 76,
Issue 10,
pp. 2080-2085,
11/15/1990
Copyright © 1990 by The American Society of Hematology
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