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Induction of human monocyte to macrophage maturation in vitro by 1,25-
dihydroxyvitamin D3
M Kreutz and R Andreesen
Medizinische Klinik der Albert-Ludwig-Universitat, Freiburg, Germany.
Cells of the mononuclear phagocyte system arise from circulating blood
monocytes (MO) that undergo further maturation on leaving the vasculature
and migration into the various tissues and body cavities. This terminal
differentiation step is also observed in vitro when blood MO are cultured
in the presence of serum. Yet, the inducing signals present in serum are
not defined. We have established primary cultures from elutriation-purified
blood MO and found that the active metabolite of vitamin D3
1,25-dihydroxyvitamin D3 (1,25(OH)2D3) could induce maturation of MO to
macrophages (MAC) in the absence of any serum proteins. Cells were cultured
for 7 days with AB-group serum or 1,25(OH)2D3, respectively, and MO
maturation analyzed by morphology, functional activity, and the expression
of lineage-restricted maturation-associated antigens (MAX.1, MAX.3). At an
optimal concentration of 10(-8) mol/L, 1,25(OH)2D3 promoted the development
of fully differentiated MAC whose phenotype and functional competence in
terms of cytokine release (tumor necrosis factor alpha, interleukin-6,
fibronectin, and lysozyme) was comparable with MAC grown in serum. In
conclusion, our data may add to the immunoregulatory potential of
1,25(OH)2D3, which may play an essential role in the ontogeny of the
mononuclear phagocyte system.
Volume 76,
Issue 12,
pp. 2457-2461,
12/15/1990
Copyright © 1990 by The American Society of Hematology
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