|
|
 Previous Article | Table of Contents | Next Article 
Lack of evidence for infection of or effect on growth of hematopoietic
progenitor cells after in vivo or in vitro exposure to human
immunodeficiency virus
JM Molina, DT Scadden, M Sakaguchi, B Fuller, A Woon and JE Groopman
Division of Hematology/Oncology, New England Deaconess Hospital, Boston, MA
02215.
The pathogenesis of the hematologic abnormalities commonly observed in
patients with acquired immunodeficiency syndrome (AIDS) is incompletely
understood. We report here that in vitro growth of myeloid (CFU-GM) and
erythroid (BFU-E) progenitor cells from six patients with AIDS was not
significantly different from that of normal human immunodeficiency virus
(HIV) seronegative donors: 25.3 +/- 5 CFU-GM per 5 x 10(4) low density
marrow cells and 33.5 +/- 5 BFU-E were observed in AIDS patients versus
32.7 +/- 5 CFU-GM and 42.1 +/- 5 BFU-E in controls. Furthermore, no HIV-DNA
in individual colonies (CFU-GM and BFU-E) could be detected using the
polymerase chain reaction (PCR) technique, although HIV-1 DNA was detected
in peripheral blood mononuclear cells from the same patients. Similarly,
normal bone marrow cells exposed in vitro to different isolates of HIV or
recombinant purified HIV-1 envelope glycoprotein (gp) 120 did not exhibit
any difference in growth of CFU-GM or BFU-E as compared with mock exposed
bone marrow cells. HIV- 1 DNA could not be detected by the PCR technique in
individual colonies derived from HIV exposed marrow. This study suggests
that committed myeloid and erythroid progenitors from AIDS patients are
responsive to hematopoietic growth factors in vitro and do not appear to
contain HIV- 1 DNA. Also, HIV or its envelope gp did not alter the growth
of hematopoietic progenitor cells in vitro. No evidence of HIV infection of
progenitor cells could be demonstrated. Impaired hematopoiesis in patients
with AIDS may not be related to direct effects of HIV on committed
progenitor cells.
Volume 76,
Issue 12,
pp. 2476-2482,
12/15/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. D. Redd, A. Avalos, and M. Essex
Infection of hematopoietic progenitor cells by HIV-1 subtype C, and its association with anemia in southern Africa
Blood,
November 1, 2007;
110(9):
3143 - 3149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamakami, M. Honda, M. Takei, Y. Ami, N. Kitamura, S. Nishinarita, S. Sawada, and T. Horie
Early Bone Marrow Hematopoietic Defect in Simian/Human Immunodeficiency Virus C2/1-Infected Macaques and Relevance to Advance of Disease
J. Virol.,
October 15, 2004;
78(20):
10906 - 10910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishii, M. Nishihara, F. Ma, Y. Ebihara, K. Tsuji, S. Asano, T. Nakahata, and T. Maekawa
Expression of Stromal Cell-Derived Factor-1/Pre-B Cell Growth-Stimulating Factor Receptor, CXC Chemokine Receptor 4, on CD34+ Human Bone Marrow Cells Is a Phenotypic Alteration for Committed Lymphoid Progenitors
J. Immunol.,
October 1, 1999;
163(7):
3612 - 3620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aiuti, L. Turchetto, M. Cota, A. Cipponi, A. Brambilla, C. Arcelloni, R. Paroni, E. Vicenzi, C. Bordignon, and G. Poli
Human CD34+ Cells Express CXCR4 and Its Ligand Stromal Cell-Derived Factor-1. Implications for Infection by T-Cell Tropic Human Immunodeficiency Virus
Blood,
July 1, 1999;
94(1):
62 - 73.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Ruiz, C. Cicala, J. Arthos, A. Kinter, A. T. Catanzaro, J. Adelsberger, K. L. Holmes, O. J. Cohen, and A. S. Fauci
Peripheral Blood-Derived CD34+ Progenitor Cells: CXC Chemokine Receptor 4 and CC Chemokine Receptor 5 Expression and Infection by HIV
J. Immunol.,
October 15, 1998;
161(8):
4169 - 4176.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Binder, M. F. van den Broek, D. Kagi, H. Bluethmann, J. Fehr, H. Hengartner, and R. M. Zinkernagel
Aplastic Anemia Rescued by Exhaustion of Cytokine-secreting CD8+ T Cells in Persistent Infection with Lymphocytic Choriomeningitis Virus
J. Exp. Med.,
June 1, 1998;
187(11):
1903 - 1920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moses, J. Nelson, and G. C. Bagby Jr
The Influence of Human Immunodeficiency Virus-1 on Hematopoiesis
Blood,
March 1, 1998;
91(5):
1479 - 1495.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. F. Weichold, D. Zella, O. Barabitskaja, J. P. Maciejewski, D. E. Dunn, E. M. Sloand, and N. S. Young
Neither Human Immunodeficiency Virus-1 (HIV-1) nor HIV-2 Infects Most-Primitive Human Hematopoietic Stem Cells as Assessed in Long-Term Bone Marrow Cultures
Blood,
February 1, 1998;
91(3):
907 - 915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Carr, H. Ramshaw, P Li, and C. Burrell
CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co-receptors and are susceptible to infection with M- and T-tropic HIV
J. Gen. Virol.,
January 1, 1998;
79(1):
71 - 75.
[Abstract]
|
|
|
|
|
|
|
I. Bahner, K. Kearns, S. Coutinho, E. H. Leonard, and D. B. Kohn
Infection of Human Marrow Stroma by Human Immunodeficiency Virus-1 (HIV-1) Is Both Required and Sufficient for HIV-1-Induced Hematopoietic Suppression In Vitro: Demonstration by Gene Modification of Primary Human Stroma
Blood,
September 1, 1997;
90(5):
1787 - 1798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Junker, J. J. Moon, C. S. Kalfoglou, I. Sniecinski, S. J. Forman, J. A. Zaia, H. Kaneshima, and E. Bohnlein
Hematopoietic Potential and Retroviral Transduction of CD34+Thy-1+ Peripheral Blood Stem Cells From Asymptomatic Human Immunodeficiency Virus Type-1-Infected Individuals Mobilized With Granulocyte Colony-Stimulating Factor
Blood,
June 15, 1997;
89(12):
4299 - 4306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Deichmann, R. Kronenwett, and R. Haas
Expression of the Human Immunodeficiency Virus Type-1 Coreceptors CXCR-4 (fusin, LESTR) and CKR-5 in CD34+ Hematopoietic Progenitor Cells
Blood,
May 15, 1997;
89(10):
3522 - 3528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. Muench, M. G. Roncarolo, and R. Namikawa
Phenotypic and Functional Evidence for the Expression of CD4 by Hematopoietic Stem Cells Isolated From Human Fetal Liver
Blood,
February 15, 1997;
89(4):
1364 - 1375.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Binder, J. Fehr, H. Hengartner, and R. M. Zinkernagel
Virus-induced Transient Bone Marrow Aplasia: Major Role of Interferon-alpha /beta during Acute Infection with the Noncytopathic Lymphocytic Choriomeningitis Virus
J. Exp. Med.,
February 3, 1997;
185(3):
517 - 530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ruiz, J Freeman, J. Bouhasin, A. Knutsen, and M. Hendrix
Arrest of in vitro T cell differentiation of normal bone marrow-derived CD34+ stem cells with thymic epithelial fragments from children with AIDS
Stem Cells,
September 1, 1996;
14(5):
533 - 547.
[Abstract]
|
|
|
|
|
