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Time-dependent alterations in the deformability of human neutrophils in
response to chemotactic activation
RS Frank
Department of Mechanical Engineering, University of Rochester, NY 14627.
Transit times of individual human neutrophils through single capillary-
sized pores were measured to determine the time-dependent changes in the
mechanical behavior of the cells during activation by the chemotactic agent
formyl-methionyl-leucyl-phenylalanine (FMLP) and in response to
cytochalasin B (CTB) and colchicine. FMLP elicited a two- phase response
consisting of a rapid increase in cell stiffness, which peaked between 2
and 3 minutes, followed by a partial recovery of deformability to a level
significantly above that of control after 5 minutes. The mechanical changes
closely followed changes in F-actin content, although the peak in cell
stiffness appeared to lag the F- actin response. Treatment with 3 mumols/L
CTB produced a transient decrease in cell rigidity followed by a return to
control level in 10 minutes, whereas treatment with 30 mumols/L CTB
resulted in a sustained decrease in cell transit times to a level 60% of
control. Addition of 3 mumols/L CTB to cells prestimulated with FMLP
produced a rapid (1 to 2 minutes) cessation of changes in cellular
deformability produced by the FMLP. Colchicine treatment did not decrease
cell rigidity, but produced a delayed increase in F-actin content
accompanied by increased stiffness of the cells. These results implicate
actin as the major determinant of the mechanical behavior of the
neutrophil, as measured by whole cell deformability tests. The significant
changes in cell deformability that occur in response to fractional changes
in F-actin content suggest that changes in the structure of the actin
network occur during these processes.
Volume 76,
Issue 12,
pp. 2606-2612,
12/15/1990
Copyright © 1990 by The American Society of Hematology
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