|
|
 Previous Article | Table of Contents | Next Article 
Impaired transendothelial migration by neonatal neutrophils: abnormalities
of Mac-1 (CD11b/CD18)-dependent adherence reactions
DC Anderson, R Rothlein, SD Marlin, SS Krater and CW Smith
Department of Pediatrics, Baylor College of Medicine, Houston, TX.
In order to evaluate the functions of lymphocyte function antigen-1 (LFA-1)
(CD11a/CD18) and Mac-1 (CD11b/CD18) on neonatal neutrophils, we examined
neutrophil adhesion to and migration through human umbilical vein
endothelial cell (HUVEC) monolayers in vitro. Transendothelial migration of
adult neutrophils was greatly enhanced by preincubation of HUVEC with
interleukin-1 (IL-1). This migration was significantly inhibited by
monoclonal antibodies (MoAbs) against LFA-1 (CD11a) and Mac-1 (CD11b)
subunits. Migration of neonatal neutrophils was markedly diminished
compared to adult neutrophils, and MoAbs against LFA-1 further reduced
migration. In contrast, anti-Mac-1 MoAb was not inhibitory. Adhesion of
adult neutrophils was significantly enhanced by prestimulation of HUVEC
with IL-1, and was significantly inhibited by MoAbs against LFA-1. Adhesion
of neonatal neutrophils was near adult levels and comparably inhibited by
anti-LFA-1 MoAb. In addition, adhesion of neonatal and adult neutrophils to
purified ICAM-1 in artificial planar membranes was comparable and almost
completely inhibited by anti-LFA-1 MoAb. Chemotactic stimulation induced
Mac-1- dependent adhesion of adult neutrophils to endothelial cells,
purified intercellular adherence molecule-1 (ICAM-1) and protein-coated
glass. In marked contrast, adhesion of neonatal neutrophils to these
substrates was not significantly increased by chemotactic stimulation.
These findings indicate that diminished transendothelial migration by
neonatal neutrophils is related to abnormal interactions of Mac-1 with
ICAM-1 and possibly other endothelial ligands. These functional deficits
may contribute to impaired inflammation and infectious susceptibility in
human neonates.
Volume 76,
Issue 12,
pp. 2613-2621,
12/15/1990
Copyright © 1990 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. W. Storm, M. M. Mariscalco, and M. F. Tosi
Postnatal maturation of total cell content and up-regulated surface expression of Mac-1 (CD11b/CD18) in polymorphonuclear leukocytes of human infants
J. Leukoc. Biol.,
August 1, 2008;
84(2):
477 - 479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Zirlik, C. Maier, N. Gerdes, L. MacFarlane, J. Soosairajah, U. Bavendiek, I. Ahrens, S. Ernst, N. Bassler, A. Missiou, et al.
CD40 Ligand Mediates Inflammation Independently of CD40 by Interaction With Mac-1
Circulation,
March 27, 2007;
115(12):
1571 - 1580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Urlichs and C. P. Speer
Neutrophil Function in Preterm and Term Infants
NeoReviews,
October 1, 2004;
5(10):
e417 - e430.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Wetzel, T. Chavakis, K. T. Preissner, M. Sticherling, U.-F. Haustein, U. Anderegg, and A. Saalbach
Human Thy-1 (CD90) on Activated Endothelial Cells Is a Counterreceptor for the Leukocyte Integrin Mac-1 (CD11b/CD18)
J. Immunol.,
March 15, 2004;
172(6):
3850 - 3859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Gerik, S. E. Keeney, D. V. Dallas, K. H. Palkowetz, and F. C. Schmalstieg
Neutrophil Adhesion Molecule Expression in the Developing Neonatal Rat Exposed to Hyperoxia
Am. J. Respir. Cell Mol. Biol.,
October 1, 2003;
29(4):
506 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Nupponen, E. Pesonen, S. Andersson, A. Makela, R. Turunen, H. Kautiainen, and H. Repo
Neutrophil Activation in Preterm Infants Who Have Respiratory Distress Syndrome
Pediatrics,
July 1, 2002;
110(1):
36 - 41.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Nupponen, S. Andersson, A.-L. Jarvenpaa, H. Kautiainen, and H. Repo
Neutrophil CD11b Expression and Circulating Interleukin-8 as Diagnostic Markers for Early-Onset Neonatal Sepsis
Pediatrics,
July 1, 2001;
108(1):
e12 - 12.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. P. Orchekowski, J. Plescia, D. C. Altieri, and M. L. Bajt
{alpha}M{beta}2 (CD11b/CD18, Mac-1) integrin activation by a unique monoclonal antibody to {alpha}M I domain that is divalent cation-sensitive
J. Leukoc. Biol.,
November 1, 2000;
68(5):
641 - 649.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S.-M. Tan, R. H. Hyland, A. Al-Shamkhani, W. A. Douglass, J. M. Shaw, and S. K. A. Law
Effect of Integrin {beta}2 Subunit Truncations on LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) Assembly, Surface Expression, and Function
J. Immunol.,
September 1, 2000;
165(5):
2574 - 2581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Lorant, W. Li, N. Tabatabaei, M. K. Garver, and K. H. Albertine
P-Selectin Expression by Endothelial Cells Is Decreased in Neonatal Rats and Human Premature Infants
Blood,
July 15, 1999;
94(2):
600 - 609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. McGuire and M. L. Bajt
Distinct Ligand Binding Sites in the I Domain of Integrin alpha(M)beta(2) That Differentially Affect a Divalent Cation-dependent Conformation
J. Biol. Chem.,
October 27, 1995;
270(43):
25866 - 25871.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
