The C gamma 1-encoded disulphide-linked and the C gamma 2-encoded
nondisulphide-linked forms of the gamma/delta heterodimer use different
gamma and delta variable regions [see comments]
M Fagioli, E Ciccone, C Bottino, B Falini, F Grignani, A Moretta, L Moretta and PG Pelicci
Istituto di Clinica Medica I, University of Perugia, Policlinico Monteluce,
Italy.
We identified two types of gamma/delta T-cell clones: (1) BB3+ clones,
which express the disulphide-linked form of the gamma/delta heterodimer
(type 1 receptor) and carry rearrangements of the C gamma 1 gene; and (2)
delta TCS1+ clones, which express the nondisulphide-linked form of the
gamma/delta heterodimer (type 2 receptor) and carry rearrangements of the C
gamma 2 gene. To determine the joining (J) and variable (V) region usage of
the two receptor types, T gamma and T delta gene rearrangements and
expression were analyzed in each gamma/delta T-cell clone. The results
showed that the J and V regions used by the two receptor types are
distinct: clones that express the type 1 receptor carry potentially
functional rearrangements in J gamma P-V gamma 9 for gamma and in J delta
1-V delta 2 for delta; and clones that express the type 2 receptor had
potentially functional rearrangements in J gamma 2- V gamma 2/V gamma 4 for
gamma and in J delta 1-V delta 1 for delta. Therefore, two clearly
different gamma/delta receptors (V gamma 9-J gamma P-C gamma 1/V delta 2-J
delta 1-C delta and V gamma 2/V gamma 4-J gamma 2-C gamma 2/V delta 1-J
delta 1-C delta) predominate in peripheral blood.
Volume 76,
Issue 2,
pp. 279-284,
07/15/1990
Copyright © 1990 by The American Society of Hematology
