Relationship between globin mRNA accumulation and commitment to terminal
cell differentiation in inducer sensitive and resistant erythroleukemia
variants
N Weich, PA Marks and RA Rifkind
DeWitt Wallace Research Laboratories; Memorial Sloan-Kettering Cancer
Center, New York, NY 10021.
The relationship between the kinetics of commitment to terminal cell
differentiation and the rates of accumulation of globin mRNA has been
examined during the induction of erythroid differentiation by polar/apolar
chemical inducers in murine erythroleukemia cells (MELC), under conditions
of more and less rapid commitment. Two differentiation inducers and three
MELC variants have been studied. Hexamethylene bisacetamide (HMBA)
initiates more rapid commitment than does dimethylsulfoxide (Me2SO). MELC
variant DR10 is resistant to induction by Me2SO and responds sluggishly to
HMBA, in comparison with the DS19- Sc9 variant. V3.17, an MELC variant
resistant to low concentrations of vincristine, shows increased sensitivity
to the inducers and an accelerated rate of commitment to terminal
differentiation compared with DS19-Sc9. It is demonstrated that commitment
and the actual expression of differentiation, as measured by the
accumulation of alpha- , beta maj-, and beta min-globin mRNA, are
temporally coordinated functions during induced differentiation of a
transformed cell line by exposure to polar/apolar agents.
Volume 76,
Issue 2,
pp. 302-306,
07/15/1990
Copyright © 1990 by The American Society of Hematology
