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Evidence that chronic lymphocytic leukemia B lymphocytes are frequently
committed to production of natural autoantibodies
L Borche, A Lim, JL Binet and G Dighiero
Immunohematologie et Immunopathologie, Institut Pasteur, Paris, France.
CD5+ B lymphocytes have been postulated to be primarily involved in
autoantibody production. To investigate whether CD5 B lymphocytes from
chronic lymphocytic leukemia (CLL) patients display the same function, we
fused B lymphocytes from 23 CD5+ B-CLL with nonsecreting murine myeloma
X-63 cells. Hybrids were derived from 18 of the 23 patients, but only
hybrids from 13 patients were found to secrete immunoglobulin (Ig) (IgM
kappa 4, IgM lambda 8, and only kappa light chain 1). Supernatants of these
hybrids were tested against an extensive panel of antigens by enzyme-linked
immunosorbent assay, indirect immunofluorescence, and hemagglutination. At
the same time, peripheral blood mononuclear cells (PBMC) from 15 of these
patients, including most patients from whom secreting hybrids had not been
obtained, were stimulated with phorbol myristate acetate (PMA). Our results
indicated that secreting heterohybrids from CLL B lymphocytes are
frequently obtained; however, this is highly dependent on the light chain
phenotype, since 8 of 9 lambda-expressing CLL produced hybrids secreting
complete Igs, as compared with 4 of 12 kappa-expressing CLL. In addition, B
lymphocytes of most patients from whom we failed to derive secreting
hybrids also failed to secrete Ig on PMA stimulation. Secondly, CD5+ B-CLL
lymphocytes were frequently committed to the production of natural
autoantibodies, since in 8 of 15 cases (including hybrids and PMA
stimulation) anti-Fc activity was found; three of these also displayed a
multispecific binding pattern.
Volume 76,
Issue 3,
pp. 562-569,
08/01/1990
Copyright © 1990 by The American Society of Hematology
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