Establishment and characterization of a granulocyte-macrophage colony-
stimulating factor-dependent human myeloid cell line
S Oez, H Tittelbach, R Fahsold, R Schaetzl, C Buhrer, J Atzpodien and JR Kalden
Medizinische Klinik III, Universitat Erlangen-Nurnberg, FRG.
A new human myeloid cell line has been established recently from the bone
marrow cells of a patient with chronic myelogenous leukemia in blast
crisis. The active proliferation and survival of the cells in RPMI 1640
medium containing fetal calf serum are clearly dependent on the presence of
either natural or recombinant human granulocyte- macrophage
colony-stimulating factor (rhGM-CSF). Despite permanent culturing in
rhGM-CSF (100 U/mL), the cells do not differentiate and bear the
myelomonocytic surface markers CD34, CD13, CD36, as well as HLA-DR, but not
CD3, CD7, CD10, CD11b, CD14, CD20, or CD42b. The predominant karyotype,
apart from tetraploidy in several cells, is 45, XX, -9, -17, -19, -22, 7p-,
9q+ (der t[9;22]), der (13q), with three additional marker chromosomes,
from which one was observed in the patient's leukemic cells. On
BglII-digested DNA, Southern blot analysis with bcr 5' as the probe
detected two additional hybridizing restriction fragments of 8.6 and 11.0
kilobase pairs.
Volume 76,
Issue 3,
pp. 578-582,
08/01/1990
Copyright © 1990 by The American Society of Hematology
