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An analysis of megakaryocytopoiesis in the C3H mouse: an animal model whose
megakaryocytes have 32N as the modal DNA class
CW Jackson, SA Steward, PJ Chenaille, RA Ashmun and TP McDonald
Department of Hematology-Oncology, St. Jude Children's Research Hospital,
Memphis 38101.
The modal DNA content of normal marrow megakaryocytes from species so far
examined usually has been reported to be 16N. In this report we describe an
exception in the C3H mouse whose megakaryocytes have a modal DNA content of
32N. Female C3H/HEN mice had an average DNA content distribution of 14% 8N,
37% 16N, 43% 32N, and 6% 64N. Male C3H/HEN mice had somewhat higher
proportions of 32N and 64N megakaryocytes (average DNA content distribution
of 12% 8N, 29% 16N, 47% 32N, and 12% 64N) than females. All 11 other mouse
strains examined had 16N as the modal megakaryocyte DNA content, although
the proportions in the various polyploid DNA classes showed some strain
variation. Megakaryocyte size was similar among all 12 strains evaluated,
and mean platelet volume (MPV) of C3H/HEN mice differed from only 1 of the
other 4 strains analyzed. Platelet counts of C3H/HEN mice were similar to
those of six, and slightly but significantly lower than those of five other
mouse strains examined. Compared with megakaryocyte concentrations of other
mouse strains studied, that of C3H/HEN mice was similar to seven, somewhat
higher than one, and slightly lower than three strains. Offspring from
reciprocal matings of C57BL/6 and C3H/HEN mice had megakaryocyte DNA
distributions intermediate between those of the parent strains, suggesting
that a higher gene dosage of some component is responsible for the
right-shifted megakaryocyte DNA content distribution phenotype of C3H mice.
The proportions of 32N and 64N megakaryocytes increased in C3H/HEN mice in
response to acute thrombocytopenia, as did those of CBA/CAJ mice used as a
comparative strain. In summary, megakaryocytes of the C3H mouse have a
higher average DNA content but similar platelet count, MPV, and
megakaryocyte size and concentration as those of most other mouse strains.
These results suggest that the number of platelets produced per unit of C3H
megakaryocyte DNA is less than that for other mice.
Volume 76,
Issue 4,
pp. 690-696,
08/15/1990
Copyright © 1990 by The American Society of Hematology
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