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RE Smith and DL Daleke
Department of Chemistry, Indiana University, Bloomington 47405.
Phosphatidylserine transport in normal and Rhnull red blood cells was
determined by measuring characteristic morphologic changes induced by
synthetic phospholipids. Treating normal A+ cells with commercial anti- A
antisera, anti-Rho(D) antisera, or with saturating concentrations of
purified Rho(D) antibodies had no effect on phosphatidylserine transport.
Normal B- cells treated with purified anti-B antibodies transported
phosphatidylserine at rates equal to those of cells not treated with
antibody. Rhnull cells, deficient in the protein bearing the Rho(D)
antigen, incorporated dimyristoylphosphatidylcholine and
dimyristoylphosphatidylserine at rates and to extents similar to normal
cells. Furthermore, incorporated phosphatidylserine, but not
phosphatidylcholine, was rapidly transported across the membrane bilayer.
Energy depletion or treatment with sulfhydryl reagents inhibited
phosphatidylserine transport equally in normal and Rhnull cells. These
results indicate that, although Rhnull cells have numerous membrane
defects, they are capable of adenosine triphosphate-dependent transport of
exogenously added dimyristoylphosphatidylserine. Normal phosphatidylserine
transport in the presence of anti-Rho(D) antibodies or in cells deficient
in the Rho(D) polypeptide indicates that this protein is not the
aminophospholipid transporter.
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| Copyright © 1990 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
