Thrombolytic properties of staphylokinase
O Matsuo, K Okada, H Fukao, Y Tomioka, S Ueshima, M Watanuki and M Sakai
Department of Physiology, Kinki University School of Medicine, Osakasayama,
Japan.
We evaluated the properties of recombinant staphylokinase in comparison
with those of tissue-type plasminogen activator (t-PA) and streptokinase
(SK). The presence of fibrin(ogen) fragment FCB-2 in the reaction mixture
increased plasminogen activation by staphylokinase more than 20-fold. Such
characteristics are similar to those of t-PA. On the other hand, SK was not
affected by the presence of FCB-2. The thrombolytic properties of
staphylokinase were studied in a system consisting of a radioactive human
plasma clot (125I-fibrinogen-labeled) suspended in the circulating citrated
plasma. Significant thrombolysis (50% in 3 hours) was obtained with 2
micrograms/mL of staphylokinase and 4.45 micrograms/mL t-PA, as compared
with 12 micrograms/mL for SK. The relative molar potency of staphylokinase,
calculated from the molecular weight, was about two times more effective
than that of SK, but about half of that of t-PA. Systemic fibrinolytic
activation and fibrinogen breakdown was not observed with staphylokinase or
t-PA, but was observed with SK. The thrombolytic efficiency of
staphylokinase, which was calculated as the ratio of the degree of
thrombolysis/the degree of fibrinogenolysis, was about five times greater
than that of SK, and about half of that of t-PA. These findings suggest
that staphylokinase has higher specific thrombolytic properties and lesser
fibrinogenolytic properties than those of SK.
Volume 76,
Issue 5,
pp. 925-929,
09/01/1990
Copyright © 1990 by The American Society of Hematology
